Del Piero F, Stremme D W, Habecker P L, Cantile C
University of Pennsylvania, School of Veterinary Medicine, New Bolton Center, 382 West Street Road, Kennett Square, PA 19348, USA.
Vet Pathol. 2006 Jan;43(1):58-61. doi: 10.1354/vp.43-1-58.
A 12-year-old male harbor seal presented with progressive signs of neurologic dysfunction including head tremors, muzzle twitching, clonic spasms, and weakness. Lesions included polioencephalomyelitis with glial nodules, spheroids, neuronophagia, ring hemorrhages, and a few neutrophils. Neurons, fibers, and glial nodules were multifocally colonized with intracytoplasmic West Nile flavivirus antigens that were demonstrated using indirect immunohistochemical analysis. Flavivirus on cultured cells also was isolated and was identified by use of monoclonal antibodies and reverse transcriptase-polymerase chain reaction analysis. Clinical signs of disease and lesion morphology and distribution were similar to those of equine West Nile virus infection. Similar to horses, alpacas, humans, dogs, and reptiles, seals can be dead-end hosts of West Nile virus.
一只12岁的雄性斑海豹出现进行性神经功能障碍症状，包括头部震颤、口鼻抽搐、阵挛性痉挛和虚弱。病变包括脑脊髓灰质炎，伴有神经胶质结节、球状体、噬神经元现象、环状出血和少量中性粒细胞。使用间接免疫组织化学分析证实，神经元、纤维和神经胶质结节多处被细胞质内西尼罗河黄病毒抗原定植。还从培养细胞中分离出黄病毒，并通过单克隆抗体和逆转录-聚合酶链反应分析进行鉴定。疾病的临床症状以及病变的形态和分布与马的西尼罗河病毒感染相似。与马、羊驼、人类、狗和爬行动物一样，海豹可能是西尼罗河病毒的终末宿主。
