Kim Eun Ok, Joo Chul Hyun, Ye Jeong Sook, Jun Eun Jung, Lee Hui Sun, Min Won-Ki, Lee Moo-Song, Lee Heuiran, Kim Yoo Kyum
Department of Microbiology, University of Ulsan College of Medicine, Seoul, South Korea.
Intervirology. 2006;49(4):192-9. doi: 10.1159/000090788. Epub 2006 Jan 11.
We investigated the clinical features, pathologic changes, and viral RNA kinetics in the course of acute and subacute experimental coxsackievirus B3 (CVB3) infection in a murine model.
Five-week-old A/J inbred male mice were divided into 5 groups. Four of those groups were inoculated intraperitoneally with 5 x 10(4) (group 1), 1 x 10(5) (group 2), 5 x 10(5) (group 3), or 1 x 10(6) (group 4) PFU of CVB3. Control mice were inoculated with uninfected Vero cell lysate in DMEM. Mice from each group were sacrificed on days 7 or 14 after inoculation.
Bloody diarrhea, earlier weight loss, perianal swelling, and death were correlated with higher viral load. One of ten mice in group 3 and 5 of 10 mice in group 4 died spontaneously between days 4 and 12 after inoculation. All of the remaining 34 mice of infected groups demonstrated extensive pancreatic inflammation. Focal myocarditis developed in only 4 (11.8%) of those 34 subjects. Amylase and creatine kinase activities in the serum were increased in the mice of infected groups. CVB3 RNA was detected in the heart and pancreatic tissue in all subjects. The CVB3 RNA copy number in pancreatic tissue was not correlated with the severity of inflammation.
In the murine model, viral loading dose determines the clinical features of CVB3-induced infection, and the severity of pancreatitis is not correlated with the viral loading dose or tissue level of viral RNA. .
我们在小鼠模型中研究了急性和亚急性实验性柯萨奇病毒B3（CVB3）感染过程中的临床特征、病理变化及病毒RNA动力学。
将5周龄的A/J近交系雄性小鼠分为5组。其中4组腹腔注射5×10⁴（第1组）、1×10⁵（第2组）、5×10⁵（第3组）或1×10⁶（第4组）空斑形成单位（PFU）的CVB3。对照组小鼠注射不含病毒的DMEM中Vero细胞裂解液。每组小鼠在接种后第7天或第14天处死。
血性腹泻、早期体重减轻、肛周肿胀和死亡与较高病毒载量相关。第3组10只小鼠中有1只、第4组10只小鼠中有5只在接种后第4天至第12天之间自发死亡。感染组其余34只小鼠均表现出广泛的胰腺炎症。这34只小鼠中仅4只（11.8%）发生局灶性心肌炎。感染组小鼠血清淀粉酶和肌酸激酶活性升高。所有受试对象的心脏和胰腺组织中均检测到CVB3 RNA。胰腺组织中CVB3 RNA拷贝数与炎症严重程度无关。
在小鼠模型中，病毒载量决定CVB3诱导感染的临床特征，胰腺炎的严重程度与病毒载量或病毒RNA的组织水平无关。
