Okada I, Matsumori A, Kyu B
Department of Internal Medicine, Faculty of Medicine, Kyoto University, Japan.
Int J Exp Pathol. 1992 Dec;73(6):721-31.
The presence of the viral RNA in the myocardium in experimental coxsackievirus B3 myocarditis of mice was investigated using the polymerase chain reaction (PCR). Four-week-old C3H/He mice (n = 35) were inoculated with coxsackievirus B3 (Nancy strain, 10(5) plaque-forming units/mouse). We used a pair of primers, which encompass a part of the 5' end sequence of the coxsackievirus B3 genome and can also detect many enteroviral RNAs. We found that hearts were positive for the viral RNA from 2 to 21 days after virus inoculation by PCR, but negative after day 28 and in non-infected control mice (n = 5). The viral RNA were detected by PCR later than by culture. Thus, the detection of the viral RNA using enzymatic amplification is more rapid and easier and may be more useful for clinical diagnosis of viral myocarditis than conventional culture methods. However, virus persistence in the myocardium long after virus inoculation is unusual in this model.
利用聚合酶链反应（PCR）研究了小鼠实验性柯萨奇病毒B3心肌炎心肌中病毒RNA的存在情况。将四周龄的C3H/He小鼠（n = 35）接种柯萨奇病毒B3（南希株，10⁵ 蚀斑形成单位/小鼠）。我们使用了一对引物，其涵盖柯萨奇病毒B3基因组5'端序列的一部分，并且还能检测许多肠道病毒RNA。我们发现，通过PCR检测，在病毒接种后2至21天心脏中病毒RNA呈阳性，但在第28天后以及未感染的对照小鼠（n = 5）中呈阴性。通过PCR检测到病毒RNA的时间比培养法要晚。因此，与传统培养方法相比，使用酶促扩增检测病毒RNA更快、更容易，可能对病毒性心肌炎的临床诊断更有用。然而，在该模型中，病毒接种后很长时间病毒仍持续存在于心肌中是不常见的。
