Schwudke Dominik, Oegema Jeffrey, Burton Lyle, Entchev Eugeni, Hannich J Thomas, Ejsing Christer S, Kurzchalia Teymuras, Shevchenko Andrej
MPI of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany.
Anal Chem. 2006 Jan 15;78(2):585-95. doi: 10.1021/ac051605m.
Data-dependent acquisition of MS/MS spectra from lipid precursors enables to emulate the simultaneous acquisition of an unlimited number of precursor and neutral loss scans in a single analysis. This approach takes full advantage of rich fragment patterns in tandem mass spectra of lipids and enables their profiling by complex (Boolean) scans, in which masses of several fragment ions are considered within a single logical framework. No separation of lipids is required, and the accuracy of identification and quantification is not compromised, compared to conventional precursor and neutral loss scanning.
基于数据的脂质前体MS/MS谱图采集能够在单次分析中模拟同时采集无限数量的前体和中性丢失扫描。这种方法充分利用了脂质串联质谱中丰富的碎片模式，并通过复杂（布尔）扫描对其进行分析，即在单个逻辑框架内考虑多个碎片离子的质量。与传统的前体和中性丢失扫描相比，无需对脂质进行分离，且不影响鉴定和定量的准确性。
