Kuwayama Masashige, Vajta Gábor, Ieda Shoko, Kato Osamu
Kato Ladies Clinic, Nishishinjuku, Shinjuku, Tokyo, Japan.
Reprod Biomed Online. 2005 Nov;11(5):608-14. doi: 10.1016/s1472-6483(10)61169-8.
Survival and development of human embryos was compared following slow cooling versus vitrification involving more than 13,000 vitrified embryos. In addition, the efficacy of an open system, the Cryotop, and a closed vitrification system, the CryoTip(trade mark), were compared using human blastocysts. One hundred percent of vitrified human pronuclear stage embryos survived and 52% developed to blastocysts as compared with 89% survival and 41% blastocyst development after slow cooling. Similar survival rates were seen with vitrification of 4-cell embryos (98%) as compared with slow cooling (91%). Furthermore, 90% of vitrified blastocysts survived and resulted in a 53% pregnancy rate following transfer, as compared with 84% survival and 51% pregnancy rates following slow cooling. All corresponding values were significantly different. When the closed and open vitrification systems were compared, no difference was found with regard to supporting blastocyst survival (93 and 97% for CryoTip and Cryotop respectively), pregnancies (51 versus 59% respectively) and deliveries (48 versus 51% respectively). Vitrification is a simple, efficient and cost-effective way to improve cumulative pregnancy rates per cycle. The use of the closed CryoTip system eliminates the potential for embryo contamination during cryopreservation and storage without compromising survival and developmental rates in vitro and in vivo.
对超过13000个经玻璃化冷冻的人类胚胎进行慢速冷冻与玻璃化冷冻后，比较了人类胚胎的存活与发育情况。此外，使用人类囊胚比较了开放式系统Cryotop和封闭式玻璃化冷冻系统CryoTip（商标）的效果。经玻璃化冷冻的人类原核期胚胎100%存活，52%发育为囊胚，而慢速冷冻后存活率为89%，囊胚发育率为41%。4细胞期胚胎玻璃化冷冻后的存活率（98%）与慢速冷冻后的存活率（91%）相似。此外，经玻璃化冷冻的囊胚90%存活，移植后的妊娠率为53%，而慢速冷冻后的存活率为84%，妊娠率为51%。所有相应数值均有显著差异。比较封闭式和开放式玻璃化冷冻系统时，在支持囊胚存活（CryoTip和Cryotop分别为93%和97%）、妊娠（分别为51%和59%）及分娩（分别为48%和51%）方面未发现差异。玻璃化冷冻是提高每个周期累积妊娠率的一种简单、高效且经济有效的方法。使用封闭式CryoTip系统可消除冷冻保存和储存过程中胚胎污染的可能性，且不影响体外和体内的存活及发育率。
