Yamasaki Masahiro, Morimoto Taisuke, Tsuji Masahito, Akihiro Iwama, Maekawa Yoshinori, Nakamura Hiroshi
Department of Endodontics, School of Dentistry, Aichi-Gakuin University, 2-11 Suemori-dori, Chikusa-ku, Nagoya 464-8651, Japan.
J Endod. 2006 Jan;32(1):24-9. doi: 10.1016/j.joen.2005.10.006.
The purpose of this study was to examine the role of IL-2 and helper T-lymphocytes in the development of periradicular lesions in rats. In control animals, periradicular lesions developed within 28 days following pulpal infection. Immunologically, some anti IL-2 and anti CD4-reactive helper T-lymphocytes infiltrated the periapical tissue at 14 days, and their numbers increased at 28 days. In experimental animals, tacrolimus (FK506) was injected every day to inhibit the IL-2 production by helper T-cells. Histologically, the pulpal necrosis and periradicular inflammation in tacrolimus-treated rats were more severe than those in the control rats. Furthermore, the areas of pulpal necrosis and periradicular lesion in the immunosuppressed rat were significantly greater than those in the normal ones. The numbers of IL-2- and CD4-positive cells in the lesion of the experimental rats were statistically lower than those of the control ones. These results show that the decrease in IL-2 might have promoted the development of periradicular lesions.
本研究的目的是探讨白细胞介素-2（IL-2）和辅助性T淋巴细胞在大鼠根尖周病变发生发展中的作用。在对照动物中，牙髓感染后28天内出现根尖周病变。在免疫方面，一些抗IL-2和抗CD4反应性辅助性T淋巴细胞在14天时浸润根尖周组织，其数量在28天时增加。在实验动物中，每天注射他克莫司（FK506）以抑制辅助性T细胞产生IL-2。组织学检查显示，他克莫司治疗的大鼠牙髓坏死和根尖周炎症比对照大鼠更严重。此外，免疫抑制大鼠的牙髓坏死面积和根尖周病变面积明显大于正常大鼠。实验大鼠病变中IL-2和CD4阳性细胞的数量在统计学上低于对照大鼠。这些结果表明，IL-2的减少可能促进了根尖周病变的发展。
