Wilson P D, Franks L M
Adv Exp Med Biol. 1975;53:171-83. doi: 10.1007/978-1-4757-0731-1_13.
The ultrastructure of perfused livers and of mitochondrial fractions from 6 months and 30 month-old C57/BL mice were studied. In old mice the liver cell mitochondria were enlarged and rounded with a light "foamy", vacuolated matrix, short cristae and a loss of dense granules. Quantitative studies showed a 60% increase in the mean size and an increased proportion of larger mitochondria in intact 30 month-old perfused livers. Endothelial and Kupffer cell mitochondria were smaller than those of the parenchymal cells. Mitochondria in pellets prepared from 6 and 30 month-old livers were rounded and condensed although there were a few larger and "foamy" mitochondria in the preparations from old mice. Up to 47% of large mitochondria in the old livers were lost during cell fractionation. The levels of cytochrome oxidase and malate dehydrogenase were slightly decreased with age but their cytochemical localization was unchanged.
研究了6个月和30个月大的C57/BL小鼠灌注肝脏及线粒体组分的超微结构。老年小鼠的肝细胞线粒体增大且呈圆形，基质轻度“泡沫状”、空泡化，嵴短，致密颗粒缺失。定量研究表明，完整的30个月大灌注肝脏中，线粒体平均大小增加60%，较大线粒体的比例增加。内皮细胞和库普弗细胞的线粒体比实质细胞的线粒体小。从6个月和30个月大肝脏制备的沉淀中的线粒体呈圆形且浓缩，尽管老年小鼠制备物中有一些较大的“泡沫状”线粒体。老年肝脏中高达47%的大线粒体在细胞分级分离过程中丢失。细胞色素氧化酶和苹果酸脱氢酶的水平随年龄略有下降，但其细胞化学定位未变。
