Collot M, Wattiaux-De Coninck S, Wattiaux R
Eur J Biochem. 1975 Feb 21;51(2):603-8. doi: 10.1111/j.1432-1033.1975.tb03962.x.
We have investigated the effect of the centrifugation speed on the behavior of rat-liver mitochondria during isopycnic centrifugation in an isoosmotic medium. The gradient was made with a macromolecular compound, glycogen dissolved in 0.25 M aqueous sucrose. The distribution curves of several mitochondrial enzymes change when the centrifugation reaches a certain speed: they are shifted toward regions of lower density. The results are plausibly explained by supposing that the inner mitochondrial membrane becomes permeable to sucrose at high centrifugation speeds, and that the granules swell. The main causal agent of the phenomenon is the hydrostatic pressure the mitochondria are subjected to during centrifugation. Morphological observations show that mitochondria are markedly deteriorated when centrifuged at high speed in the glycogen gradient; they are swollen and the outer membrane is broken; also frequently, a large electron-dense granule is seen in the matrix near the inner mambrane.
我们研究了离心速度对大鼠肝脏线粒体在等渗介质中进行等密度离心时行为的影响。梯度是用一种大分子化合物——溶解于0.25M蔗糖水溶液中的糖原制成的。当离心达到一定速度时，几种线粒体酶的分布曲线会发生变化：它们会向低密度区域移动。通过假设线粒体内膜在高离心速度下对蔗糖变得可渗透，且颗粒膨胀，这些结果得到了合理的解释。该现象的主要成因是线粒体在离心过程中所承受的静水压力。形态学观察表明，当在糖原梯度中高速离心时，线粒体明显受损；它们肿胀且外膜破裂；此外，在内膜附近的基质中也经常能看到一个大的电子致密颗粒。
