[巢蛋白和神经生成素3在人胎儿胰腺中的表达]
[Expression of nestin and neurogenin 3 in the human fetal pancreas].
作者信息
Zheng Zong-mei, Chen Dong-ming, Li Ling-song, Li Jian-ning, Shen Li, Lu Ai-li, Wang Shu-ling, Bao Wei-han
机构信息
Department of Plastic Surgery, Peking University Third Hospital, Beijing 100083, China.
出版信息
Zhonghua Wai Ke Za Zhi. 2005 Dec 1;43(23):1537-40.
OBJECTIVE
To examine the expression of nestin and neurogenin 3 (Ngn3), the markers of pancreatic stem cells, in the human fetal pancreas.
METHODS
The human fetal pancreas tissue of 12 and 14 weeks were examined for the expression of nestin and Ngn3 using the techniques of immunofluorescence dye and RT-PCR.
RESULTS
Both nestin and Ngn3 expressed widely in 12 and 14 weeks before in human fetal pancreatic tissue. In these positive cells there was no co-expressing insulin or glucagon. There were nestin and Ngn3 co-expressing cells in ducts but not in the islets. The results of RT-PCR also indicated the expression of nestin and Ngn3.
CONCLUSIONS
There was no expression of the markers of mature endocrine cells in the nestin and Ngn3 positive cells, and they were the marks of no-differentiation cells in the human fetal pancreatic tissue.
目的
检测胰腺干细胞标志物巢蛋白（nestin）和神经生成素3（Ngn3）在人胎儿胰腺中的表达。
方法
采用免疫荧光染色和逆转录-聚合酶链反应（RT-PCR）技术，检测12周和14周人胎儿胰腺组织中nestin和Ngn3的表达。
结果
nestin和Ngn3在人胎儿胰腺组织12周和14周时均广泛表达。在这些阳性细胞中，没有胰岛素或胰高血糖素的共表达。导管中有nestin和Ngn3共表达细胞，但胰岛中没有。RT-PCR结果也表明了nestin和Ngn3的表达。
结论
nestin和Ngn3阳性细胞中未表达成熟内分泌细胞标志物，它们是人胎儿胰腺组织中未分化细胞的标志物。
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