基于胶束介质中鲁米诺-K2Cr2O7化学发光的流动注射法测定痕量腺嘌呤。

Flow injection determination of adenine at trace level based on luminol-K2Cr2O7 chemiluminescence in a micellar medium.

作者信息

Erbao Liu, Bingchun Xue

机构信息

College of Chemistry and Life Sciences, Tianjin Normal University, Tianjin 300074, PR China.

出版信息

J Pharm Biomed Anal. 2006 May 3;41(2):649-53. doi: 10.1016/j.jpba.2005.12.012. Epub 2006 Jan 18.

DOI:10.1016/j.jpba.2005.12.012

PMID:16413727

Abstract

A novel flow injection chemiluminescence (CL) analysis method for the determination of adenine in the presence of sodium dodecylbenzene sulfonate (SDBS) surfactant micelles is described. This method is based on the luminescent properties of the luminol-K2Cr2O7-adenine in alkaline medium sensitized by SDBS. The optimized experimental conditions were evaluated and the possible mechanism was discussed by examining CL emission spectrum and the effect of various free radical scavengers on CL emission intensity. The CL increase is linearly related to the concentration of adenine in the range of 2.92x10(-6) to 4.38x10(-10) mol l-1 with a detection limit of 2.46x10(-10) mol l-1 (S/N=3). The relative standard deviation for 2.92x10(-7) mol l-1 samples was 1.67% (n=12). The proposed method has been applied to the determination of adenine in human serum.

摘要

描述了一种用于测定在十二烷基苯磺酸钠（SDBS）表面活性剂胶束存在下腺嘌呤的新型流动注射化学发光（CL）分析方法。该方法基于在SDBS敏化的碱性介质中鲁米诺 - K2Cr2O7 - 腺嘌呤的发光特性。通过检查CL发射光谱和各种自由基清除剂对CL发射强度的影响，评估了优化的实验条件并讨论了可能的机制。CL的增加与腺嘌呤浓度在2.92×10（-6）至4.38×10（-10）mol l-1范围内呈线性关系，检测限为2.46×10（-10）mol l-1（S/N = 3）。2.92×10（-7）mol l-1样品的相对标准偏差为1.67%（n = 12）。所提出的方法已应用于测定人血清中的腺嘌呤。