Gómez-Taylor B, Palomeque M, García Mateo J V, Martínez Calatayud J
Departamento de Ciencias Químicas, Universidad Cardenal Herrera CEU, Moncada (Valencia), Spain.
J Pharm Biomed Anal. 2006 May 3;41(2):347-57. doi: 10.1016/j.jpba.2005.11.040. Epub 2006 Jan 18.
A screening test for the forward development of chemiluminescence systems able to determine pharmaceutical compounds is reported. The test is based on the on-line photodegradation of the drugs by using a photoreactor consisting of 697 cmx0.5 mm PTFE tubing helically coiled around an 8 W low-pressure mercury lamp. Photodegraded pharmaceuticals are detected by direct chemiluminescence of the resulting photofragments and their subsequent reaction with potassium permanganate in sulphuric acid medium as oxidant. The screening comprised 97 compounds with different molecular structures and relevant members of the most important families of pharmaceuticals are tested (amino acids, carboxylic acids, nitrocompounds, phenyl-alkyl and aromatic amines, sulphonic acid amides, polycarbocyclics, monocyclic N-containing heterocyclics, bicyclic N-containing heterocyclics, tricyclic N-containing heterocyclics, N-S containing heterocyclics...). Due to the relevant influence of the medium for the photodegradation a wide range of pH's and buffer solutions were studied. The proposed strategy (photoinduced chemiluminescence, Ph-CL) allows the development of systems for the determination of many pharmaceuticals which do not present "native" chemiluminescence (e.g. chloramphenicol, dextromethorpham, riboflavin, ephedrine, piperazinamide, chlotrimazole, theophylline...). Moreover, Ph-CL allows to increase the sensitivity of chemiluminescence procedures based on direct chemiluminescence detection (e.g. sulphonamides, thiazides, nicontinamide, nortryptiline, levamisole, phenylbarbituric acid...).
报道了一种用于筛选能够测定药物化合物的化学发光系统正向发展的测试方法。该测试基于药物的在线光降解，使用由697厘米×0.5毫米的聚四氟乙烯管螺旋缠绕在一个8瓦低压汞灯周围组成的光反应器。通过所得光碎片的直接化学发光及其随后在硫酸介质中与作为氧化剂的高锰酸钾反应来检测光降解的药物。筛选包括97种具有不同分子结构的化合物，并测试了最重要药物家族的相关成员（氨基酸、羧酸、硝基化合物、苯基烷基和芳香胺、磺酸酰胺、多环化合物、单环含氮杂环化合物、双环含氮杂环化合物、三环含氮杂环化合物、含氮硫杂环化合物……）。由于介质对光降解有重要影响，研究了广泛的pH值和缓冲溶液。所提出的策略（光致化学发光，Ph-CL）允许开发用于测定许多不具有“天然”化学发光的药物的系统（例如氯霉素、右美沙芬、核黄素、麻黄碱、哌嗪酰胺、克霉唑、茶碱……）。此外，Ph-CL允许提高基于直接化学发光检测的化学发光程序的灵敏度（例如磺胺类药物、噻嗪类药物、烟酰胺、去甲替林、左旋咪唑、苯巴比妥酸……）。
