Departamento de Ciencias Quimicas, Universidad Cardenal Herrera CEU, Moncada, Valencia, Spain.
Talanta. 2003 Jun 13;60(2-3):405-14. doi: 10.1016/S0039-9140(03)00074-2.
A new, simple, clean and selective flow injection strategy based on the tandem photochemical reaction-chemiluminescence detection was applied to the determination of chloramphenicol. The determination is based on the on-line photodegradation of the drug in a glycine buffer at pH 8.8 by using a photoreactor consisting of 697 cmx0.5 mm PTFE tubing helically coiled around an 8 W low-pressure mercury lamp. Photodegradated chloramphenicol is detected by direct chemiluminescence of resulting photo-fragments and their subsequent reaction with potassium permanganate in sulfuric acid medium as oxidant. The method allows the chemiluminescence determination of compounds which do not exhibit native chemiluminescence. The calibration graph was linear up to 14 mug ml(-1) chloramphenicol, the limit of detection was 30 ng ml(-1), the relative standard deviation was 2.4% for 7 mug ml(-1) of the drug and the sample throughput was 60 h(-1). Taking into account the importance of the medium of photodegradation on the mechanism of photodegradation a comparative study in terms of selective was performed for different chemical media employed in the procedure of photodegradation. The proposed method was applied to the determination of chloramphenicol in commercially available pharmaceutical formulations.
一种新的、简单的、清洁的和选择性的基于串联光化学反应-化学发光检测的流动注射策略被应用于氯霉素的测定。该测定是基于在 pH 值为 8.8 的甘氨酸缓冲液中，药物的在线光降解，使用由 697 cmx0.5 mm PTFE 管螺旋缠绕在 8 W 低压汞灯下组成的光反应器。光降解的氯霉素通过所得光碎片的直接化学发光及其随后与硫酸介质中的高锰酸钾的反应来检测。该方法允许对不具有天然化学发光的化合物进行化学发光测定。校准曲线在线性范围内可达 14 微克毫升(-1)氯霉素，检测限为 30 纳克毫升(-1)，7 微克毫升(-1)药物的相对标准偏差为 2.4%，样品通量为 60 小时(-1)。考虑到光降解介质对光降解机制的重要性，在光降解过程中采用不同化学介质进行了选择性的比较研究。该方法被应用于市售药物制剂中氯霉素的测定。
