Larralde Osmany, Smith Richard W P, Wilkie Gavin S, Malik Poonam, Gray Nicola K, Clements J Barklie
MRC Human Genetics Unit, Western General Hospital, Edinburgh EH4 2XU, Scotland, United Kingdom.
J Virol. 2006 Feb;80(3):1588-91. doi: 10.1128/JVI.80.3.1588-1591.2006.
Herpes simplex virus type 1 (HSV-1) ICP27 protein is an essential regulator of viral gene expression with roles at various levels of RNA metabolism in the nucleus. Using the tethered function assay, we showed a cytoplasmic activity for ICP27 in directly enhancing mRNA translation in vivo in the absence of other viral factors. The region of ICP27 required for translational stimulation maps to the C terminus. Furthermore, in infected cells, ICP27 is associated with polyribosomes, indicating a function in translation during the lytic cycle.
单纯疱疹病毒1型（HSV-1）的ICP27蛋白是病毒基因表达的重要调节因子，在细胞核内RNA代谢的各个层面发挥作用。利用拴系功能检测法，我们发现，在没有其他病毒因子的情况下，ICP27在体内直接增强mRNA翻译方面具有胞质活性。翻译刺激所需的ICP27区域定位于C末端。此外，在受感染的细胞中，ICP27与多核糖体相关联，这表明它在裂解周期中具有翻译功能。
