Lu Yuan, Liu Xi-shi, Wang Yue-xiang, Song Hou-yan, Zhong Nanbert
Department of Gynecology, The Hospital of Obstetrics and Gynecology, Fudan University, Shanghai 200011, China.
Beijing Da Xue Xue Bao Yi Xue Ban. 2006 Feb 18;38(1):62-5.
To evaluate the frequency of MSI in epithelial ovarian tumors and its relationship with clinicopathologic features.
Ninety fresh specimens of epithelial ovarian tumors, including 74 primary and 16 secondary tumors, were collected. Microsatellite analysis was carried out using 5 mono-and dinucleotide markers from the National Cancer Institute Consensus Panel by fluorescence-labeled polymerase chain reaction.
Of 90 epithelial ovarian tumors analyzed, 18 demonstrated a high level of microsatellite instability (MSI-H), 30 demonstrated a low level of microsatellite instability (MSI-L), and the remaining 42 exhibited microsatellite stability (MSS). Frequency of microsatellite instability (MSI) at loci BAT-25 was higher than that at any other loci. No correlation was found between MSI level and patient age, tumor type, tumor differentiation (P>0.05). But the microsatellite instability-high phenotype correlates with clinical stage. It tended to occur more frequently in early-stage tumors (P=0.03).
The frequent MSI in epithelial ovarian tumors suggests that it is an early event to involve in the development of epithelial ovarian tumors.
评估上皮性卵巢肿瘤中微卫星不稳定性（MSI）的频率及其与临床病理特征的关系。
收集90例上皮性卵巢肿瘤新鲜标本，其中包括74例原发性肿瘤和16例继发性肿瘤。采用美国国立癌症研究所共识小组的5个单核苷酸和二核苷酸标记，通过荧光标记聚合酶链反应进行微卫星分析。
在分析的90例上皮性卵巢肿瘤中，18例表现为高度微卫星不稳定（MSI-H），30例表现为低度微卫星不稳定（MSI-L），其余42例表现为微卫星稳定（MSS）。BAT-25位点的微卫星不稳定性（MSI）频率高于其他任何位点。未发现MSI水平与患者年龄、肿瘤类型、肿瘤分化之间存在相关性（P>0.05）。但微卫星高度不稳定表型与临床分期相关。它在早期肿瘤中更易发生（P=0.03）。
上皮性卵巢肿瘤中频繁出现的MSI表明其是上皮性卵巢肿瘤发生发展过程中的早期事件。
