Teal P E A, Proveaux A T
Center for Medical, Agricultural and Veterinary Entomology, USDA-ARS, Gainesville, Florida 32604, USA.
Arch Insect Biochem Physiol. 2006 Feb;61(2):98-105. doi: 10.1002/arch.20104.
Gas chromatographic-mass spectral analysis of extracts obtained from in vitro culture of isolated retrocerebral complexes obtained from adult females of the moth Heliothis virescens resulted in identification of methyl farnesoate as well as juvenile hormone III (JH III) but not JH III acid. Inhibition of JH biosynthesis by incubation of tissue in synthetic Manduca sexta allatostatin (Manse-AST, pGlu-Val-Arg-Phe-Arg-Gln-Cys-Tyr-Phe-Asn-Pro-Ile-Ser-Cys-Phe-COOH) reduced production of these chemicals to negligible levels. However, incubation of tissue in the presence of Manse-AST plus farnesol resulted in production of significant amounts of both methyl farnesoate and JH III. Tissue incubated in the presence of Manse-AST plus methyl farnesoate produced only JH III. The results indicated that methyl farnesoate is naturally produced by the corpora allata of adult females of Heliothis virescens. However, tissue incubated in the presence of Manse-AST plus JH III acid also produced JH III in amounts equivalent to that produced by tissue incubated with methyl farnesoate. Thus, both methyl farnesoate and JH III acid could serve as a precursor for biosynthesis of JH III.
对从烟草天蛾成年雌性个体分离得到的脑后复合体进行体外培养所获提取物的气相色谱 - 质谱分析，结果鉴定出了法尼酸甲酯以及保幼激素III（JH III），但未检测到JH III酸。通过在合成的烟草天蛾咽侧体抑制素（Manse - AST，pGlu - Val - Arg - Phe - Arg - Gln - Cys - Tyr - Phe - Asn - Pro - Ile - Ser - Cys - Phe - COOH）中孵育组织来抑制JH生物合成，可将这些化学物质的产量降低到可忽略不计的水平。然而，在Manse - AST加法尼醇存在的情况下孵育组织，会产生大量的法尼酸甲酯和JH III。在Manse - AST加甲基法尼酯存在的情况下孵育的组织仅产生JH III。结果表明，法尼酸甲酯是烟草天蛾成年雌性咽侧体天然产生的。然而，在Manse - AST加JH III酸存在的情况下孵育的组织也产生了与在甲基法尼酯孵育的组织中产生量相当的JH III。因此，法尼酸甲酯和JH III酸都可以作为JH III生物合成的前体。
