Ochel Hans-Joachim
Clinic for Radiation Therapy, Radiobiological Laboratory, Medical Faculty, Otto-von-Guericke University, Leipziger Str. 44, 39120 Magdeburg, Germany.
J Exp Ther Oncol. 2005;5(1):9-13.
Radiobiological experiments routinely require the estimation of cellular survival. Several cytotoxicity assays have been proposed, which have inherent advantages and disadvantages. The performance of one of them, the crystal violet dissolution assay, was examined under a broad range of conditions. PC-3M prostate carcinoma cells were exposed to ionizing radiation in the presence or absence of heat shock protein 90-inhibiting drugs. Colony formation as assessed by traditional manual counting was compared with the results from the crystal violet dissolution assay. The overall bivariate correlation of the data was determined (r = 0.743, p < 0.01). The variabilities of the data distributions from two different observers were quantitated and found to agree fairly. Ionizing radiation has a scattering-reducing effect that if plotted as Pearson-coeffcient versus radiation energy dose yields a sigmoidal distribution of data points. The crystal violet dissolution assay is a convenient tool for the rapid and reproducible determination of cellular survival and under appropriate conditions exerts a reasonable correlation to colony number.
放射生物学实验通常需要估算细胞存活率。已经提出了几种细胞毒性检测方法，这些方法都有其固有的优缺点。其中一种方法，即结晶紫溶解检测法，在广泛的条件下进行了性能检测。将PC-3M前列腺癌细胞在有或没有热休克蛋白90抑制药物的情况下暴露于电离辐射。将通过传统手工计数评估的集落形成与结晶紫溶解检测法的结果进行比较。确定了数据的总体双变量相关性（r = 0.743，p < 0.01）。对来自两名不同观察者的数据分布变异性进行了定量，发现相当一致。电离辐射具有散射减少效应，如果将其绘制为皮尔逊系数与辐射能量剂量的关系图，则会得到数据点的S形分布。结晶紫溶解检测法是一种用于快速、可重复测定细胞存活率的便捷工具，在适当条件下与集落数量具有合理的相关性。
