辛伐他汀对 DU145 前列腺癌细胞放射敏感性的调节作用。
Modulation of radiosensitivity of DU145 prostate carcinoma cells by simvastatin.
机构信息
Laboratory for Radiation Biology, Clinic for Radiotherapy, Medical Faculty, Otto-von-Guericke-University, Magdeburg, Germany.
Radiation Oncology, Universitätsklinikum Magdeburg, Leipziger Str. 44, DE 39120, Magdeburg, Germany.
出版信息
J Cancer Res Clin Oncol. 2023 Jul;149(8):4509-4514. doi: 10.1007/s00432-022-04364-9. Epub 2022 Sep 21.
PURPOSE
To investigate antiproliferative effects of simvastatin in combination with ionizing radiation on DU145 prostate cancer cells and its influence on cellular HMG-CoA-reductase levels.
METHODS
Proliferative responses of DU145 cells were estimated by means of a clonogenic assay or the crystal violet procedure. HMG-CoA-reductase levels were measured by western blot analysis.
RESULTS
The antiproliferative effects of simvastatin and radiation are dependent on simvastatin dose, radiation dose and treatment time. In vitro treatment of DU145 cells with simvastatin induced HMG-CoA-reductase levels.
CONCLUSION
Ionizing radiation more profoundly reduces proliferation as compared to simvastatin exposure, while the combined application of both modalities is synergistic. The inhibition of CoA-reductase may contribute to these effects.
目的
研究辛伐他汀联合电离辐射对 DU145 前列腺癌细胞的增殖抑制作用及其对细胞 HMG-CoA 还原酶水平的影响。
方法
通过集落形成实验或结晶紫法评估 DU145 细胞的增殖反应。通过 Western blot 分析测量 HMG-CoA 还原酶水平。
结果
辛伐他汀和辐射的增殖抑制作用取决于辛伐他汀剂量、辐射剂量和治疗时间。体外用辛伐他汀处理 DU145 细胞诱导 HMG-CoA 还原酶水平升高。
结论
与辛伐他汀暴露相比，电离辐射更能显著降低增殖，而两种方式的联合应用具有协同作用。CoA 还原酶的抑制可能有助于这些作用。
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