Saleh Yousif, Sebzda Tadeusz, Warwas Maria, Kopec Wieslaw, Ziólkowska Jolanta, Siewinski Maciej
Department of Forensic Medicine, Molecular Technical Unit, Wroclaw Medical University, Curie-Sklodowskiej 52, 50-369 Wroclaw, Poland.
J Exp Ther Oncol. 2005;5(1):49-53.
We studied the relation between the antipapain activity of cysteine proteinase inhibitors (CPI) and immunohistochemical staining for cystatin C, using anti-chicken cystatin antibodies, in the colorectal cancer tissues. In primary tumour tissues immuno-peroxidase reactivity was present in the cytoplasm and on the cell surface membranes. Sections of non malignant tissues showed no staining. The percentages of positive staining were greater for adenocarcinoma than carcinoma,100% and 77% respectively. Antipapain activity which was increased in malignant tissues in comparison to control, rose successively from well differentiated carcinomas through moderately to poor differentiated. Invasive adenocarcinomas had higher antipapain activity than noninvasive ones. The results indicated that immunohistochemical detection of cystatin using anti-chicken cystatin antibodies could be useful in studying the prognostic significance of cystatin C expression in colorectal cancer.
我们使用抗鸡半胱氨酸蛋白酶抑制剂抗体，研究了结直肠癌组织中半胱氨酸蛋白酶抑制剂（CPI）的抗木瓜蛋白酶活性与胱抑素C免疫组化染色之间的关系。在原发性肿瘤组织中，免疫过氧化物酶反应性存在于细胞质和细胞表面膜上。非恶性组织切片未显示染色。腺癌的阳性染色百分比高于癌，分别为100%和77%。与对照相比，恶性组织中增加的抗木瓜蛋白酶活性从高分化癌依次升高至中分化癌和低分化癌。浸润性腺癌的抗木瓜蛋白酶活性高于非浸润性腺癌。结果表明，使用抗鸡半胱氨酸蛋白酶抑制剂抗体对胱抑素进行免疫组化检测，可能有助于研究胱抑素C在结直肠癌中表达的预后意义。
