Shur I, Socher R, Benayahu D
Department of Cell and Developmental Biology, Sackler School of Medicine, Tel-Aviv University, Tel-Aviv, Israel.
J Cell Physiol. 2006 May;207(2):374-8. doi: 10.1002/jcp.20586.
Molecular mechanisms that control cell differentiation involve with chromatin remodeling activities. We recently identified Chromatin Related Mesenchymal Modulator (CReMM), a CHD protein expressed by mesenchymal cells. In this study, we analyzed CReMM expression on RNA and protein levels during embryonic development in mouse skeletal tissues. CReMM appears transiently during mesenchymal cell differentiation, being detected first in osteoprogenitors and declining in mature cells. A novel aspect of the study elaborates on in vivo association of CReMM with promoters in cells obtained by laser capture micro-dissection (LCM) technique from periosteum and endochondreal ossification regions. Using chromatin immunoprecipitation (ChIP), we proved that CReMM binds to skeletal tissue-specific promoters: CBFA1, biglycan, osteocalcin (OC), collagen-II, and myosin in a differential manner. The results imply that CReMM selectively interacts with analyzed promoters activated in the tissue at the appropriate time of development. The identification of CReMM and its tissue distribution and function provides an attractive clue for the study of transcriptional regulation of osteogenic cells' maturation.
控制细胞分化的分子机制与染色质重塑活动有关。我们最近鉴定出染色质相关间充质调节剂（CReMM），一种由间充质细胞表达的CHD蛋白。在本研究中，我们分析了小鼠骨骼组织胚胎发育过程中CReMM在RNA和蛋白质水平上的表达。CReMM在间充质细胞分化过程中短暂出现，首先在骨祖细胞中被检测到，在成熟细胞中下降。该研究的一个新方面阐述了通过激光捕获显微切割（LCM）技术从骨膜和软骨内成骨区域获得的细胞中CReMM与启动子的体内关联。使用染色质免疫沉淀（ChIP），我们证明CReMM以不同方式与骨骼组织特异性启动子：CBFA1、双糖链蛋白聚糖、骨钙素（OC）、胶原蛋白II和肌球蛋白结合。结果表明CReMM在发育的适当时间与组织中激活的分析启动子选择性相互作用。CReMM的鉴定及其组织分布和功能为研究成骨细胞成熟的转录调控提供了一个有吸引力的线索。
