Yajima Ichiro, Belloir Elodie, Bourgeois Yveline, Kumasaka Mayuko, Delmas Véronique, Larue Lionel
Developmental Genetics of Melanocytes, UMR 146 CNRS-Institut Curie, Centre Universitaire, Orsay, France.
Genesis. 2006 Jan;44(1):34-43. doi: 10.1002/gene.20182.
The organ-specific and temporal control of gene activation/inactivation is a key issue in the understanding of protein function during normal and pathological development and during oncogenesis. We generated transgenic mice bearing a tamoxifen-dependent Cre recombinase (Tyr::Cre-ERT2) gene expressed under the control of a 6.1 kb murine tyrosinase promoter in order to facilitate targeted spatiotemporally controlled somatic recombination in melanoblasts/melanocytes. Cre-ERT2 production was detected in tissues containing melanocytes. After tamoxifen induction at various times during embryogenesis and adulthood in a Cre-responsive reporter mouse strain, genetic recombination was detected in the melanoblasts and melanocytes of the skin. Thus, the Tyr::Cre-ERT2 transgenic mice provides a valuable tool for following this cell lineage and for investigating gene function in melanocyte development and transformation.
基因激活/失活的器官特异性和时间控制是理解正常和病理发育过程以及肿瘤发生过程中蛋白质功能的关键问题。我们构建了转基因小鼠，其携带在6.1 kb小鼠酪氨酸酶启动子控制下表达的他莫昔芬依赖性Cre重组酶（Tyr::Cre-ERT2）基因，以便于在黑素母细胞/黑素细胞中实现靶向的时空可控体细胞重组。在含有黑素细胞的组织中检测到了Cre-ERT2的产生。在胚胎期和成年期的不同时间对Cre反应性报告基因小鼠品系进行他莫昔芬诱导后，在皮肤的黑素母细胞和黑素细胞中检测到了基因重组。因此，Tyr::Cre-ERT2转基因小鼠为追踪该细胞谱系以及研究黑素细胞发育和转化中的基因功能提供了有价值的工具。
