铜绿假单胞菌III型分泌基因表达的代谢调控
Metabolic regulation of type III secretion gene expression in Pseudomonas aeruginosa.
作者信息
Rietsch Arne, Mekalanos John J
机构信息
Department of Microbiology and Molecular Genetics, Harvard Medical School, 200 Longwood Ave., Boston, MA 02115, USA.
出版信息
Mol Microbiol. 2006 Feb;59(3):807-20. doi: 10.1111/j.1365-2958.2005.04990.x.
Abstract
Type III secretion-mediated cytotoxicity is one of the key virulence mechanisms of the opportunistic pathogen Pseudomonas aeruginosa. Prior data from several laboratories have established that metabolism is a key factor in the regulation of type III secretion gene expression in P. aeruginosa. Here we use a fluorescence-activated cell sorter (FACS)-based approach to investigate expression of type III secretion genes at a single-cell level. The data demonstrate that the metabolic state regulates the percentage of cells that are able to induce type III secretion gene expression under inducing conditions. We also present evidence that this regulation is the result of an effect of the growth conditions on the ability of P. aeruginosa to assemble a functional type III secretion apparatus. Preliminary data suggest that the metabolite that controls type III secretion gene expression is derived from acetyl-CoA and that this regulation may, in part, be mediated by changes in the intracellular concentration of cyclic-AMP.
摘要
III型分泌介导的细胞毒性是机会致病菌铜绿假单胞菌的关键毒力机制之一。几个实验室先前的数据表明，代谢是铜绿假单胞菌III型分泌基因表达调控的关键因素。在此，我们使用基于荧光激活细胞分选仪（FACS）的方法在单细胞水平上研究III型分泌基因的表达。数据表明，代谢状态调节在诱导条件下能够诱导III型分泌基因表达的细胞百分比。我们还提供证据表明，这种调节是生长条件对铜绿假单胞菌组装功能性III型分泌装置能力影响的结果。初步数据表明，控制III型分泌基因表达的代谢物源自乙酰辅酶A，并且这种调节可能部分由细胞内环磷酸腺苷浓度的变化介导。
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