Wilcks Andrea, Hansen Bjarne Munk, Hendriksen Niels Bohse, Licht Tine Rask
Department of Microbiological Food Safety, Danish Institute for Food and Veterinary Research, Mørkhøj Bygade 19, DK-2860 Søborg, Denmark.
FEMS Immunol Med Microbiol. 2006 Feb;46(1):70-7. doi: 10.1111/j.1574-695X.2005.00007.x.
The fate and effect of Bacillus cereus F4433/73R in the intestine of human-flora-associated rats was studied using bacteriological culturing techniques and PCR-denaturing gradient gel electrophoresis in combination with cell assays and immunoassays for detection of enterotoxins. In faecal samples from animals receiving vegetative cells, only few B. cereus cells were detected. Spores survived the gastric barrier well, and were in some cases detected up to 2 weeks after ingestion. Selective growing revealed no major changes in the intestinal flora during passage of B. cereus. However, denaturing gradient gel electrophoresis analysis with universal 16S rRNA gene primers revealed significant changes in the intestinal microbiota of animals dosed with spores. Vero cell assays and a commercial kit (BCET-RPLA) did not reveal any enterotoxin production from B. cereus F4433/73R in the intestinal tract.
采用细菌培养技术、聚合酶链反应-变性梯度凝胶电泳（PCR-DGGE），并结合细胞试验和免疫试验检测肠毒素，研究了蜡样芽孢杆菌F4433/73R在人源化大鼠肠道中的命运和作用。在接受营养细胞的动物粪便样本中，仅检测到少量蜡样芽孢杆菌细胞。芽孢能很好地耐受胃屏障，在某些情况下，摄入后2周仍能检测到。选择性培养显示，蜡样芽孢杆菌通过肠道期间，肠道菌群没有重大变化。然而，用通用16S rRNA基因引物进行的变性梯度凝胶电泳分析显示，接种芽孢的动物肠道微生物群有显著变化。Vero细胞试验和商用试剂盒（BCET-RPLA）未显示蜡样芽孢杆菌F4433/73R在肠道中产生任何肠毒素。
