Daniels D R, Wang J C, Briehl R W, Turner M S
Department of Physics, University of Warwick, Coventry CV4 7AL, United Kingdom.
J Chem Phys. 2006 Jan 14;124(2):024903. doi: 10.1063/1.2148960.
We give a theoretical treatment of the force exerted by a fluctuating membrane on a polymer rod tip, taking into account the effects of an underlying biological cytoskeleton by way of a simple harmonic dependence on displacement. We also consider theoretically and experimentally the dynamics of a growing fiber tip under the influence of such a fluctuation-induced membrane force, including the effects of an underlying cytoskeletal network. We compare our model with new experimental data for the growth of hemoglobin fibers within red blood cells, revealing a good agreement. We are also able to estimate the force and membrane/cytoskeletal displacement required to stall growth of, or buckle, a growing fiber. We discuss the significance of our results in a biological context, including how the properties of the membrane and cytoskeleton relate to the thermodynamics of rod polymerization.
我们对波动膜施加在聚合物杆尖端的力进行了理论处理，通过对位移的简谐依赖关系考虑了潜在生物细胞骨架的影响。我们还从理论和实验上考虑了在这种波动诱导的膜力影响下生长纤维尖端的动力学，包括潜在细胞骨架网络的影响。我们将我们的模型与红细胞内血红蛋白纤维生长的新实验数据进行了比较，结果显示吻合度良好。我们还能够估计使生长纤维停止生长或发生弯曲所需的力以及膜/细胞骨架位移。我们在生物学背景下讨论了我们结果的意义，包括膜和细胞骨架的特性如何与杆状聚合物的热力学相关。
