Van den Velde Sandra, Lagrou Katrien, Desmet Koen, Wauters Georges, Verhaegen Jan
Laboratory Medicine, Department of Microbiology and Immunology, University Hospitals Leuven, BE-3000 Leuven, Belgium.
Diagn Microbiol Infect Dis. 2006 Feb;54(2):99-104. doi: 10.1016/j.diagmicrobio.2005.08.019. Epub 2006 Jan 19.
We evaluated the usefulness of cellular fatty acid analysis for the identification of corynebacteria. Therefore, 219 well-characterized strains belonging to 21 Corynebacterium species were analyzed with the Sherlock System of MIDI (Newark, DE). Most Corynebacterium species have a qualitative different fatty acid profile. Corynebacterium coyleae (subgroup 1), Corynebacterium riegelii, Corynebacterium simulans, and Corynebacterium imitans differ only quantitatively. Corynebacterium afermentans afermentans and C. coyleae (subgroup 2) have both a similar qualitative and quantitative profile. The commercially available database (CLIN 40, MIDI) identified only one third of the 219 strains correctly at the species level. We created a new database with these 219 strains. This new database was tested with 34 clinical isolates and could identify 29 strains correctly. Strains that remained unidentified were 2 Corynebacterium aurimucosum (not included in our database), 1 C. afermentans afermentans, and 2 Corynebacterium pseudodiphtheriticum. Cellular fatty acid analysis with a self-created database can be used for the identification and differentiation of corynebacteria.
我们评估了细胞脂肪酸分析在棒状杆菌鉴定中的实用性。因此，使用MIDI公司（特拉华州纽瓦克市）的Sherlock系统对属于21种棒状杆菌的219株特征明确的菌株进行了分析。大多数棒状杆菌物种具有质的不同的脂肪酸谱。科伊尔棒状杆菌（第1亚组）、里氏棒状杆菌、模拟棒状杆菌和模仿棒状杆菌仅在量上有所不同。非发酵非发酵棒状杆菌和科伊尔棒状杆菌（第2亚组）在质和量上都具有相似的谱。市售数据库（CLIN 40，MIDI）在物种水平上仅正确鉴定了219株菌株中的三分之一。我们用这219株菌株创建了一个新数据库。用34株临床分离株对这个新数据库进行了测试，能够正确鉴定29株。仍未鉴定的菌株有2株金色粘棒状杆菌（未包含在我们的数据库中）、1株非发酵非发酵棒状杆菌和2株假白喉棒状杆菌。使用自行创建的数据库进行细胞脂肪酸分析可用于棒状杆菌的鉴定和区分。
