Reiterová K, Tomasovicová O, Dubinský P
Parasitological Institute of the Slovak Academy of Sciences, Hlinkova 3, 040 01 Kosice, Slovak Republic.
Parasitology. 2006 May;132(Pt 5):625-33. doi: 10.1017/S0031182005009741. Epub 2006 Jan 23.
The impact of Toxocara canis infection of Balb/c mice mothers on the future immune response of their offspring towards reinfection with the same parasite was studied. Two groups of offspring, the first originating from the mothers infected with a single dose of 1000 Toxocara canis eggs and the second from non-infected mothers, were both challenged with 500 T. canis eggs per animal at 6 weeks of age. The proportions of spleen CD4+ and CD8+ T lymphocytes, the level of serum cytokines IFN-gamma and IL-5, eosinophilia in peripheral blood, the production of specific antibodies and the number of migrating larvae were monitored. In both groups of offspring, the challenge infection resulted in an increase in CD4+ T-cell subtype in comparison with the non-infected healthy control, although after an initial decline a subsequent increase in CD8+ was observed. The immunoregulation index (CD4+/CD8+) was lower in the group of mice originating from infected mothers throughout the whole experiment compared to the offspring of non-infected mothers as well as in healthy control mice of the same age. In the offspring of infected mothers mainly, a reduced production of IFN-gamma and of IL-5, suppressed eosinophilia and a higher level of protective antibodies was detected, compared to the control second group, in which the INF-gamma concentration significantly increased after day 42 p.i. In the first group of offspring before challenge, 12.7 +/- 2.5 larvae in the brains and 32 +/- 2.1 larvae in the muscles transmitted from the infected mothers were detected. There was a significant reduction in larval recovery from brain on days 42 and 49 p.i. (56.7 and 56.8%, respectively), while from muscles in the same time there was a reduction of 46.7 and 39%, respectively, compared to the offspring of non-infected mothers. These results indicate a significant protective memory of immune mechanisms against T. canis induced in offspring of Toxocara-infected mother mice.
研究了犬弓首蛔虫感染Balb/c小鼠母亲对其后代未来针对同一寄生虫再感染的免疫反应的影响。两组后代，第一组来自感染单剂量1000个犬弓首蛔虫卵的母亲，第二组来自未感染的母亲，两组在6周龄时均每只动物用500个犬弓首蛔虫卵进行攻击。监测了脾脏CD4+和CD8+ T淋巴细胞的比例、血清细胞因子IFN-γ和IL-5的水平、外周血嗜酸性粒细胞增多情况、特异性抗体的产生以及迁移幼虫的数量。在两组后代中，与未感染的健康对照相比，攻击感染导致CD4+ T细胞亚型增加，尽管在最初下降后观察到CD8+随后增加。在整个实验过程中，来自感染母亲的小鼠组的免疫调节指数（CD4+/CD8+）低于未感染母亲的后代以及同龄健康对照小鼠。与第二对照组相比，在感染母亲的后代中主要检测到IFN-γ和IL-5的产生减少、嗜酸性粒细胞增多受到抑制以及保护性抗体水平较高，在第二对照组中，感染后42天IFN-γ浓度显著增加。在第一组后代攻击前，检测到从感染母亲传播来的大脑中有12.7±2.5条幼虫，肌肉中有32±2.1条幼虫。在感染后42天和49天，大脑中幼虫的回收率显著降低（分别为56.7%和56.8%），而同一时间肌肉中的幼虫回收率与未感染母亲的后代相比分别降低了46.7%和39%。这些结果表明，犬弓首蛔虫感染的母鼠后代中诱导产生了针对犬弓首蛔虫的免疫机制的显著保护性记忆。
