Foo I, Salo W L, Aufderheide A C
Department of Biochemistry, University of Minnesota, Duluth 55812.
Biotechniques. 1992 Jun;12(6):811-4, 817.
We describe a generalized PCR method that will amplify fragments of DNA without any knowledge of sequence using a single primer. Although we are presently using this method to amplify DNA fragments isolated from ancient preserved tissues, in effect, producing PCR libraries, it may prove to have other applications.
我们描述了一种通用的聚合酶链式反应（PCR）方法，该方法使用单一引物，无需任何序列信息即可扩增DNA片段。尽管我们目前正在使用这种方法扩增从古代保存组织中分离的DNA片段，实际上是在构建PCR文库，但它可能会有其他应用。
