Brüssow Klaus-Peter, Torner Helmut, Rátky Jozsef, Manabe Noboru, Tuchscherer Armin
FBN Research Institute for the Biology of Farm Animals, Dummerstorf, Germany.
J Reprod Dev. 2006 Apr;52(2):249-57. doi: 10.1262/jrd.17085. Epub 2006 Jan 20.
In the pig, a temporal relationship is suggested between sperm release from the sperm reservoir (SR) and ovulation, but the mechanism(s) is still under discussion. In two experiments, the influence of transferred ova on the release of SR-spermatozoa at ovulation and the effect of supplementation with non-sulfated glycosaminoglycan hyaluronan (HA) on embryo development and the number of accessory spermatozoa, respectively, were examined. PMSG/hCG primed ovectomized gilts that had previously received endoscopic low-dose insemination into the cranial uterine horn were used as an experimental model. After salpingectomy, tubal segments (ampulla, cranial, and caudal isthmus) were flushed and sperm numbers or respective accessory spermatozoa were counted. In Experiment 1, the distribution of the sperm population was altered in the presence of cumulus-oocyte-complexes (COCs). A higher proportion of spermatozoa was found after transfer of COCs into one oviduct in the ampulla and cranial isthmus segments compared with the controls (17.5 vs. 4.9%, p<0.05). In Experiment 2, the quality of the transferred ova and treatment influenced the presence of accessory spermatozoa. Transfer of COCs together with HA increased (p<0.05) the number of accessory spermatozoa compared with the other treatment groups and was similar to those in the "undisturbed" controls. No modifications were obtained regarding mean blastomere numbers (2.6 +/- 0.2 to 3.1 +/- 0.2). In summary, this study was demonstrated that cumulus-oocyte-complexes may be involved in triggering sperm release from the pig oviductal SR and that HA might be related to sperm release.
在猪身上，精子从精子储存库（SR）释放与排卵之间存在一种时间关系，但具体机制仍在探讨中。在两项实验中，分别研究了移植卵母细胞对排卵时SR精子释放的影响，以及补充非硫酸化糖胺聚糖透明质酸（HA）对胚胎发育和附属精子数量的影响。将预先接受内窥镜低剂量子宫角输精的孕马血清促性腺激素/人绒毛膜促性腺激素预处理的去卵巢后备母猪用作实验模型。输卵管切除术后，冲洗输卵管段（壶腹部、峡部头端和峡部尾端）并计数精子数量或相应的附属精子。在实验1中，卵丘-卵母细胞复合体（COCs）的存在改变了精子群体的分布。与对照组相比，将COCs转移到一侧输卵管的壶腹部和峡部头端段后，发现精子比例更高（17.5%对4.9%，p<0.05）。在实验2中，移植卵母细胞的质量和处理方式影响了附属精子的存在。与其他处理组相比，COCs与HA一起转移增加了（p<0.05）附属精子的数量，且与“未受干扰”的对照组相似。关于平均卵裂球数量（2.6±0.2至3.1±0.2）未获得任何改变。总之，本研究表明卵丘-卵母细胞复合体可能参与触发猪输卵管SR中的精子释放，且HA可能与精子释放有关。
