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成熟的 COC 促进壶腹 NPPC 的产生，这对于猪卵母细胞从输卵管细胞中释放精子是必需的。

The Mature COC Promotes the Ampullary NPPC Required for Sperm Release from Porcine Oviduct Cells.

机构信息

Division of Cell, Developmental and Integrative Biology, School of Medicine, South China University of Technology, Guangzhou 510006, China.

State Key Laboratory for Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, China.

出版信息

Int J Mol Sci. 2023 Feb 4;24(4):3118. doi: 10.3390/ijms24043118.

DOI:10.3390/ijms24043118
PMID:36834527
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9967908/
Abstract

Porcine spermatozoa are stored in the oviductal isthmus after natural mating, and the number of spermatozoa is increased in the oviductal ampulla when the mature cumulus-oocyte complexes (COCs) are transferred into the ampulla. However, the mechanism is unclear. Herein, natriuretic peptide type C (NPPC) was mainly expressed in porcine ampullary epithelial cells, whereas its cognate receptor natriuretic peptide receptor 2 (NPR2) was located on the neck and the midpiece of porcine spermatozoa. NPPC increased sperm motility and intracellular Ca levels, and induced sperm release from oviduct isthmic cell aggregates. These actions of NPPC were blocked by the cyclic guanosine monophosphate (cGMP)-sensitive cyclic nucleotide-gated (CNG) channel inhibitor -Diltiazem. Moreover, porcine COCs acquired the ability to promote NPPC expression in the ampullary epithelial cells when the immature COCs were induced to maturation by epidermal growth factor (EGF). Simultaneously, transforming growth factor-β ligand 1 (TGFB1) levels were dramatically increased in the cumulus cells of the mature COCs. The addition of TGFB1 promoted NPPC expression in the ampullary epithelial cells, and the mature COC-induced NPPC was blocked by the transforming growth factor-β type 1 receptor (TGFBR1) inhibitor SD208. Taken together, the mature COCs promote NPPC expression in the ampullae via TGF-β signaling, and NPPC is required for the release of porcine spermatozoa from the oviduct isthmic cells.

摘要

猪精子在自然交配后储存在输卵管峡部，当成熟的卵丘-卵母细胞复合物（COCs）转移到输卵管壶腹部时，精子数量在输卵管壶腹部增加。然而，其机制尚不清楚。在此，脑钠肽 C（NPPC）主要在猪输卵管上皮细胞中表达，而其同源受体脑钠肽受体 2（NPR2）位于猪精子的颈部和中段。NPPC 增加了精子的活力和细胞内 Ca 水平，并诱导精子从输卵管峡部细胞聚集体中释放。NPPC 的这些作用被环鸟苷单磷酸（cGMP）敏感的环核苷酸门控（CNG）通道抑制剂地尔硫卓阻断。此外，当不成熟的 COCs 被表皮生长因子（EGF）诱导成熟时，猪 COCs 获得了促进输卵管上皮细胞中 NPPC 表达的能力。同时，成熟 COCs 的卵丘细胞中转化生长因子-β配体 1（TGFB1）水平显著增加。TGFB1 的添加促进了输卵管上皮细胞中 NPPC 的表达，而 TGF-β 型 1 受体（TGFBR1）抑制剂 SD208 阻断了成熟 COC 诱导的 NPPC。总之，成熟的 COCs 通过 TGF-β 信号促进输卵管中 NPPC 的表达，NPPC 是猪精子从输卵管峡部细胞中释放所必需的。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d4aa/9967908/bf1a43ad7dac/ijms-24-03118-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d4aa/9967908/995c351c0a14/ijms-24-03118-g001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d4aa/9967908/bf1a43ad7dac/ijms-24-03118-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d4aa/9967908/995c351c0a14/ijms-24-03118-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d4aa/9967908/1f03100fec03/ijms-24-03118-g002.jpg
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