Lee Kwang-Jun, Bae Song-Mee, Lee Myoung-Ro, Yeon Sun-Mi, Lee Young-Hee, Kim Ki-Sang
Division of Bacterial Respiratory Infections, Department of Microbiology, National Institute of Health, Korea Center for Diseases Control and Prevention, Eunpyung-Ku, Seoul, Korea.
Proteomics. 2006 Feb;6(4):1274-82. doi: 10.1002/pmic.200500415.
Streptococcus pneumoniae is an important human pathogen that causes a variety of diseases, such as pneumonia, bacteremia, meningitis, otitis media, and sinusitis, in both adults and children. The global pattern of growth phase-dependent protein expression of S. pneumoniae during in vitro culture was analyzed using 2-DE combined with MALDI-TOF MS and LC/ESI-MS/MS. Several protein production patterns were observed at four time points throughout the growth stage, although some protein levels did not change significantly. We focused on the switch in protein expression at the transition from log growth phase to stationary phase. Proteins that were significantly induced or repressed at this point are likely to be involved in central intermediary metabolism, amino acid synthesis, nucleotide, and fatty acid metabolism, cell wall synthesis, protein degradation, and stress responses. This global expression profiling approach has revealed previously unrecognized relationships between proteins in the life of this pathogen.
肺炎链球菌是一种重要的人类病原体，可在成人和儿童中引起多种疾病，如肺炎、菌血症、脑膜炎、中耳炎和鼻窦炎。利用二维电泳结合基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）和液相色谱/电喷雾串联质谱（LC/ESI-MS/MS）分析了肺炎链球菌在体外培养过程中生长阶段依赖性蛋白质表达的全球模式。在整个生长阶段的四个时间点观察到了几种蛋白质产生模式，尽管一些蛋白质水平没有显著变化。我们关注对数生长期向稳定期转变时蛋白质表达的变化。在这一点上被显著诱导或抑制的蛋白质可能参与中心中间代谢、氨基酸合成、核苷酸和脂肪酸代谢、细胞壁合成、蛋白质降解和应激反应。这种全球表达谱分析方法揭示了该病原体生命过程中蛋白质之间以前未被认识的关系。
