Servicio de Microbiologia-INIBIC, Complejo Hospitalario Universitario La Coruna, As Xubias s/n, La Coruna, Spain.
J Proteome Res. 2010 Apr 5;9(4):1951-64. doi: 10.1021/pr901116r.
Acinetobacter baumannii is an opportunistic pathogen that has been associated with severe infections and outbreaks in hospitals. At present, very little is known about the biology of this bacterium, particularly as regards mechanisms of adaptation, persistence and virulence. To investigate the growth phase-dependent regulation of proteins in this microorganism, we analyzed the proteomic pattern of A. baumannii ATCC 17978 at different stages of in vitro growth. In this study, proteomics analyses were conducted using 2-DE and MALDI-TOF/TOF complemented by iTRAQ LC-MS/MS. Here we have identified 107 differentially expressed proteins. We highlight the induction of proteins associated with signaling, putative virulence factors and response to stress (including oxidative stress). We also present evidence that ROS (O(2)(-) and OH(-)) and RNI (ONOO(-)) accumulate during late stages of growth. Further assays demonstrated that stationary cells survive at high concentrations of H(2)O(2) (30 mM), the O(2)(-) donor menadione (500 muM) or the NO donor sodium nitroprusside (1 mM), and showed a higher survival rate against several bactericidal antibiotics. The growth phase-dependent changes observed in the A. baumannii proteome are discussed within a context of adaptive biological responses, including those related to ROS and RNI stress.
鲍曼不动杆菌是一种机会致病菌，与医院严重感染和爆发有关。目前，人们对这种细菌的生物学特性知之甚少，特别是关于适应、持续存在和毒力的机制。为了研究该微生物在生长阶段依赖的蛋白质调控，我们分析了在不同体外生长阶段的鲍曼不动杆菌 ATCC 17978 的蛋白质组模式。在这项研究中，我们使用 2-DE 和 MALDI-TOF/TOF 进行蛋白质组学分析，并通过 iTRAQ LC-MS/MS 进行补充。在此，我们鉴定了 107 个差异表达的蛋白质。我们强调了与信号转导、潜在毒力因子和应激反应（包括氧化应激）相关的蛋白质的诱导。我们还提供了证据表明 ROS（O2(-)和 OH(-)）和 RNI（ONOO(-)）在生长后期积累。进一步的实验表明，静止细胞可以在高浓度的 H2O2（30 mM）、O2(-)供体 menadione（500 μM）或 NO 供体 sodium nitroprusside（1 mM）中存活，并且对几种杀菌抗生素表现出更高的存活率。在鲍曼不动杆菌蛋白质组中观察到的生长阶段依赖性变化，是在适应生物反应的背景下讨论的，包括与 ROS 和 RNI 应激有关的反应。
