Taira T, Fujinaga T, Okumura M, Yamashita K, Tsunoda N, Mizuno S
Department of Veterinary Surgery, Faculty of Veterinary Medicine, Hokkaido University, Hayakita-cho, Japan.
J Vet Med Sci. 1992 Jun;54(3):435-42. doi: 10.1292/jvms.54.435.
Haptoglobin (Hp) was isolated from equine serum by ammonium sulphate precipitation, anion-exchange chromatography and gel filtration. Equine Hp which migrated to the alpha 2-globulin region in electrophoresis, contained 2 fractions with molecular weights (NW) of 108,000 and 105,000, and each fraction consisting of 2 subunits. Quantitative measurement of Hp in equine serum was performed by the single radial immunodiffusion method using anti-equine Hp serum. In clinically normal horses, the highest concentration of serum Hp was found in newborn foals and a high value was maintained until 12 months of age. The concentration then decreased with age. Normal Hp values were 5.25 +/- 2.36 mg/ml in foals (less than or equal to 12 months old), 2.19 +/- 1.54 mg/ml in adult horses (greater than or equal to 18 months old) and 3.62 +/- 0.81 mg/ml in all horses. Serum Hp concentration in mares during the perinatal period in comparison with the normal adult female was high for 4 months pre-partum, a passing increase at delivery, and then decreased at 2 weeks post-partum returning to normal within 1 month of delivery. In horses with experimentally-induced inflammation, serum Hp concentration began to increase immediately after treatment and reached the highest value, 1.5 to 9 times higher than those of pre-treatment at 2 to 5 days, then decreased within 4 weeks. It was also elevated in most cases of horses with clinically inflammatory signs.
通过硫酸铵沉淀、阴离子交换色谱法和凝胶过滤从马血清中分离出触珠蛋白（Hp）。在电泳中迁移至α2球蛋白区域的马Hp包含分子量（MW）为108,000和105,000的2个组分，且每个组分由2个亚基组成。使用抗马Hp血清通过单向放射免疫扩散法对马血清中的Hp进行定量测定。在临床正常的马匹中，血清Hp浓度在新生驹中最高，并在12月龄前保持较高水平。随后该浓度随年龄降低。驹（小于或等于12月龄）的正常Hp值为5.25±2.36mg/ml，成年马（大于或等于18月龄）为2.19±1.54mg/ml，所有马匹为3.62±0.81mg/ml。与正常成年雌性相比，母马围产期血清Hp浓度在产前4个月较高，分娩时短暂升高，然后在产后2周下降，在分娩后1个月内恢复正常。在实验性诱导炎症的马匹中，血清Hp浓度在治疗后立即开始升高，并在2至5天达到最高值，比治疗前高1.5至9倍，然后在4周内下降。在大多数有临床炎症体征的马匹中其也升高。
