Zimmermann P, Ritzmann M, Selbitz H-J, Heinritzi K, Truyen U
Institut für Mikrobiologie der Bundeswehr, Neuherbergstraße 11, 80937 München, Germany.
Klinik für Schweine, Tierärztliche Fakultät, Ludwig-Maximilians-Universität München, Sonnenstraße 16, 85764 Oberschleißheim, Germany.
J Gen Virol. 2006 Feb;87(Pt 2):295-301. doi: 10.1099/vir.0.81086-0.
In order to evaluate the genetic variability of Porcine parvovirus (PPV), the complete capsid protein sequences (VP1/VP2) from seven recent field isolates from Germany, one isolate from the UK and one German vaccine strain were sequenced and analysed, along with two American (NADL-2 and Kresse), three Asian and 22 Brazilian partial PPV sequences retrieved from GenBank. The analysis revealed a high degree of diversity: 1.2-2.6% at the nucleotide level and 1.2-6.8% at the amino acid level. Phylogenetic analysis defined two German clusters: one formed by four German isolates and the English, Asian and American sequences; and the second, distinct cluster formed by the other three of the seven German isolates examined. The latter cluster was still observed when the 22 partial sequences (853 nt of the 3' terminus of the VP2 gene) from the Brazilian isolates were included in the analyses, indicating that the VP2 sequence determines the phylogeny.
为了评估猪细小病毒（PPV）的遗传变异性，对来自德国的7株近期田间分离株、1株来自英国的分离株和1株德国疫苗株的完整衣壳蛋白序列（VP1/VP2）进行了测序和分析，同时还分析了从GenBank中检索到的2株美国毒株（NADL-2和Kresse）、3株亚洲毒株以及22株巴西PPV部分序列。分析结果显示出高度的多样性：核苷酸水平上为1.2%-2.6%，氨基酸水平上为1.2%-6.8%。系统发育分析确定了两个德国聚类：一个由4株德国分离株以及英国、亚洲和美国的序列组成；另一个不同的聚类由所检测的7株德国分离株中的另外3株组成。当将巴西分离株的22个部分序列（VP2基因3'末端的853 nt）纳入分析时，仍能观察到后一个聚类，这表明VP2序列决定了系统发育。
