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人脱矿骨基质中骨形态发生蛋白-2、-4和-7的测定

Assay of bone morphogenetic protein-2, -4, and -7 in human demineralized bone matrix.

作者信息

Pietrzak William S, Woodell-May Jennifer, McDonald Nicholas

机构信息

Biomet, Inc., Warsaw, Indiana 46581, USA.

出版信息

J Craniofac Surg. 2006 Jan;17(1):84-90. doi: 10.1097/01.scs.0000179745.91165.73.

Abstract

Demineralized bone matrix (DBM) is a widely used bone graft material that derives its osteoinductive potential from matrix-associated bone morphogenetic proteins (BMPs). Prior investigations have shown that the osteoinductive potential can vary widely, with influence from both donor and processing sources. Although it is plausible that donor variance in the BMP profile can be an important consideration, the few published studies available have given inconsistent and incomplete information about this. The goal was to (1) characterize the variance of BMP-2, BMP-4, and BMP-7 in fully demineralized DBM derived from 20 appropriately screened (Food and Drug Administration and the American Association of Tissue Banks criteria) donors (male and female, 17-65 years) and (2) using literature review, infer the potential for this to be an important source of variability in graft function. BMPs were extracted with 4 M guanidine hydrochloride, and levels of BMP-2, BMP-4, and BMP-7 were measured using enzyme-linked immunosorbent assay. Measured levels were as follows: BMP-2 = 21.4 +/- 12.0 ng/g DBM, BMP-4 = 5.45 +/- 2.04 ng/g DBM, and BMP-7 = 84.1 +/- 34.4 ng/g DBM, which were significantly different (P < 0.05). There was a positive linear correlation between BMP-2 and BMP-7 (P = 0.0227). DBM derived from female donors had significantly greater concentrations of BMP-2 and BMP-7 than did that derived from male donors (P = 0.0257 and 0.0245, respectively). There was no significant correlation between donor age and the levels of any of the measured BMPs. The magnitude of variance of BMP profile appears to reasonably well correspond to the variance in osteoinductive potential cited by others, suggesting the possibility of using this as a method of donor screening.

摘要

脱矿骨基质(DBM)是一种广泛应用的骨移植材料,其骨诱导潜能源于与基质相关的骨形态发生蛋白(BMPs)。先前的研究表明,骨诱导潜能差异很大,受供体和加工来源的影响。虽然BMP谱中的供体差异可能是一个重要考虑因素看似合理,但现有的少数已发表研究对此给出的信息并不一致且不完整。目标是:(1) 表征从20名经过适当筛选(符合美国食品药品监督管理局和美国组织银行协会标准)的供体(男性和女性,17 - 65岁)获得的完全脱矿DBM中BMP - 2、BMP - 4和BMP - 7的差异;(2) 通过文献综述,推断这有可能成为移植功能变异性的一个重要来源。用4M盐酸胍提取BMPs,采用酶联免疫吸附测定法测量BMP - 2、BMP - 4和BMP - 7的水平。测量水平如下:BMP - 2 = 21.4±12.0 ng/g DBM,BMP - 4 = 5.45±2.04 ng/g DBM,BMP - 7 = 84.1±34.4 ng/g DBM,差异有统计学意义(P < 0.05)。BMP - 2与BMP - 7之间存在正线性相关(P = 0.0227)。来自女性供体的DBM中BMP - 2和BMP - 7的浓度显著高于来自男性供体的DBM(分别为P = 0.0257和0.0245)。供体年龄与任何测量的BMP水平之间均无显著相关性。BMP谱的差异程度似乎与其他人引用的骨诱导潜能差异相当吻合,这表明有可能将此用作供体筛选的一种方法。

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