Department of Periodontics , Maulana Azad Institute of Dental Sciences , New Delhi, 110002, India.
Structural Immunology Group, International Centre for Genetic Engineering and Biotechnology, New Delhi, 110067, India.
Cell Tissue Bank. 2024 Jun;25(2):475-484. doi: 10.1007/s10561-023-10103-2. Epub 2023 Aug 14.
The presence of bone morphogenetic proteins in demineralized freeze-dried bone allograft (DFDBA) are responsible for developing hard tissues in intraosseous defects. The most common mode of sterilization of bone allografts, i.e., Gamma rays, have dramatic effects on the structural and biological properties of DFDBA, leading to loss of BMPs. Ultraviolet-C radiation is a newer approach to sterilize biodegradable scaffolds, which is simple to use and ensures efficient sterilization. However, UV-C radiation has not yet been effectively studied to sterilize bone allografts. This study aimed to compare and evaluate the effectiveness of Gamma and Ultraviolet-C rays in sterilizing indigenously prepared DFDBA and assess their effect on the quantity of BMP-7 present in the allograft. DFDBA samples from non-irradiated, gamma irradiated, and UV-C irradiated groups were tested for BMP-7 level and samples sterilized with gamma and UV-C rays were analysed for sterility testing. The estimated mean BMP-7 level was highest in non-irradiated DFDBA samples, followed by UV-C irradiated, and the lowest in gamma irradiated samples. Our study concluded that UV-C rays effectively sterilized DFDBA as indicated by negative sterility test and comprised lesser degradation of BMP-7 than gamma irradiation.
脱矿冻干骨同种异体移植物（DFDBA）中存在的骨形态发生蛋白负责在骨内缺损中形成硬组织。同种异体移植物最常见的灭菌方式，即伽马射线，对 DFDBA 的结构和生物学特性有显著影响，导致 BMP 丢失。紫外线-C 辐射是一种用于消毒可生物降解支架的较新方法，使用简单，可确保有效消毒。然而，紫外线-C 辐射尚未有效研究用于消毒同种异体移植物。本研究旨在比较和评估伽马射线和紫外线-C 射线在消毒本地制备的 DFDBA 方面的有效性，并评估它们对同种异体移植物中存在的 BMP-7 数量的影响。对未辐照、伽马辐照和紫外线-C 辐照组的 DFDBA 样本进行 BMP-7 水平检测，对经伽马和紫外线-C 射线消毒的样本进行无菌检测。未辐照 DFDBA 样本的 BMP-7 水平估计最高，其次是紫外线-C 辐照样本，伽马辐照样本最低。我们的研究得出结论，紫外线-C 射线有效地对 DFDBA 进行了消毒，如无菌检测阴性所示，并且与伽马照射相比，BMP-7 的降解较少。
