Liu Gang L, Rodriguez Victoria B, Lee Luke P
Biomolecular Nanotechnology Center, Berkeley Sensor and Actuator Center, Department of Bioengineering, University of California at Berkeley, Berkeley, CA 94720, USA.
J Nanosci Nanotechnol. 2005 Nov;5(11):1933-7. doi: 10.1166/jnn.2005.417.
Au nanoparticles have distinctive absorption spectra whose peak position or particle plasmon resonance wavelength is highly sensitive to molecule adsorption on their surfaces. Spherical Au nanoparticles are surface-modified by amino-functionalized self-assembly-monolayer and used as optical probes in the fluorescence-label-free spectroscopic detection of sub-nanomole oligonucleotides. Time-resolved studies of the immobilization and hybridization of oligonucleotides on the surface of Au nanoparticles were carried out. By measuring peak shift of absorption spectra of the Au colloidal nanoparticles over time, the samples of 15 nM 20 mer target and mismatched oligonucleotides are distinguished by their different influences on the particle plasmon resonance wavelength. The approach presented in this paper extends the application of Au nanoparticles as the optical probe in oligonucleotide recognitions without prior sample labeling.
金纳米颗粒具有独特的吸收光谱，其峰值位置或粒子等离子体共振波长对分子在其表面的吸附高度敏感。球形金纳米颗粒通过氨基功能化自组装单层进行表面修饰，并用作光学探针，用于亚纳摩尔寡核苷酸的无荧光标记光谱检测。对寡核苷酸在金纳米颗粒表面的固定和杂交进行了时间分辨研究。通过测量金胶体纳米颗粒吸收光谱的峰值随时间的变化，15 nM 20聚体靶标和错配寡核苷酸样品因其对粒子等离子体共振波长的不同影响而得以区分。本文提出的方法扩展了金纳米颗粒作为光学探针在寡核苷酸识别中的应用，无需对样品进行预先标记。
