Wöhrl Stefan, Vigl Kornelia, Binder Michael, Stingl Georg, Prinz Michael
Department of Dermatology, Division of Immunology, Allergy and Infectious Diseases (DIAID), Medical University of Vienna, Austria.
Exp Dermatol. 2006 Feb;15(2):119-24. doi: 10.1111/j.1600-0625.2006.00388.x.
Using the Khoros image processing software environment, we developed a software-based system capable of extracting wheal size from skin prick tests (SPTs) in mm(2) for research and routine purposes.
At 20 min, the outlines of up to 20 wheals were marked with a pen and transferred with translucent adhesive tapes to a white paper form carrying predefined markings. The form was scanned at 200 dpi. The software automatically analysed the scanned image and calculated the sizes of the wheals. In a pilot study, serial SPTs with histamine in increasing dilutions were performed in 12 healthy volunteers in duplicate on both volar forearms. We matched the application results with a reference created from the scanned pictures.
Bland-Altman analysis showed reference and software calculation reaching very high agreement. The comparison of reference/software resulted in a low centred coefficient of variation (COV) of 11.9%. This was superior to the conventional measurement of horizontal (COV 37.9%) or maximal/minimal diameter (COV 25.9%).
We present an accurate tool for exactly calculating SPT wheal size in mm(2).
利用Khoros图像处理软件环境，我们开发了一种基于软件的系统，能够从皮肤点刺试验（SPT）中提取以平方毫米（mm²）为单位的风团大小，用于研究和日常目的。
在20分钟时，用钢笔标记多达20个风团的轮廓，并用半透明胶带将其转移到带有预定义标记的白纸模板上。以200 dpi的分辨率扫描该模板。软件自动分析扫描图像并计算风团大小。在一项初步研究中，对12名健康志愿者的双侧前臂掌侧进行了组胺系列稀释液的SPT，一式两份。我们将应用结果与从扫描图片创建的参考值进行了匹配。
Bland-Altman分析显示参考值与软件计算结果高度一致。参考值/软件计算结果的中心变异系数（COV）较低，为11.9%。这优于传统的水平测量（COV 37.9%）或最大/最小直径测量（COV 25.9%）。
我们提出了一种精确计算以mm²为单位的SPT风团大小的准确工具。
