Srinivas N R, Igwemezie L N
Department of Metabolism and Pharmacokinetics, Bristol-Myers Squibb Company, Syracuse, New York, NY 13221.
Biomed Chromatogr. 1992 Jul-Aug;6(4):163-7. doi: 10.1002/bmc.1130060402.
The increased attention on the therapeutic implications of stereoisomerism has provided an impetus for the development of analytical methods for enantiomeric separation. The indirect method of separation of enantiomers as diastereomers using high performance liquid chromatography (HPLC) has emerged as an efficient and versatile approach. This is due mainly to the availability of numerous chiral derivatization reagents (CDRs). This article reviews CDRs useful for the development of an indirect HPLC method using ultraviolet, fluorescence and electrochemical detection. In addition, factors crucial for the development of the indirect method are discussed.
对立体异构现象治疗意义的日益关注推动了对映体分离分析方法的发展。使用高效液相色谱（HPLC）将对映体作为非对映体进行间接分离的方法已成为一种高效且通用的方法。这主要归功于大量手性衍生化试剂（CDR）的可得性。本文综述了可用于开发采用紫外、荧光和电化学检测的间接HPLC方法的手性衍生化试剂。此外，还讨论了间接方法开发的关键因素。
