Bhushan R, Ali I
Department of Chemistry, University of Roorkee, India.
Biomed Chromatogr. 1992 Jul-Aug;6(4):196-7. doi: 10.1002/bmc.1130060409.
The separation of tetracycline and amino glycopeptide antibiotics was achieved on silica gel thin layers. Tetracycline antibiotics were resolved on a Co+2 (1.0%) impregnated silica gel layer using ethanol:acetic acid:water (10:6:6, v/v/v) as the mobile phase. Amino glycopeptide antibiotics were separated on an untreated silica gel layer using the mobile phase n-butanol:formic acid:water (6:5:7, v/v/v). The spots of these antibiotics were located by exposing the chromatoplate to iodine vapours.
在硅胶薄层上实现了四环素和氨基糖肽类抗生素的分离。四环素类抗生素在以乙醇：乙酸：水（10:6:6，v/v/v）为流动相的Co²⁺（1.0%）浸渍硅胶层上得以分离。氨基糖肽类抗生素在未处理的硅胶层上以正丁醇：甲酸：水（6:5:7，v/v/v）为流动相进行分离。通过将色谱板暴露于碘蒸气中来定位这些抗生素的斑点。
