Li Dan, Borovkov Alexander, Vaglenov Alexander, Wang Chengming, Kim Teayoun, Gao Dongya, Sykes Kathryn F, Kaltenboeck Bernhard
Department of Pathobiology, College of Veterinary Medicine, Auburn University, 270 Greene Hall, Auburn, AL 36849-5519, USA.
Vaccine. 2006 Apr 5;24(15):2917-27. doi: 10.1016/j.vaccine.2005.12.035. Epub 2006 Jan 4.
An inbred A/J mouse respiratory challenge model was validated for vaccine testing against Chlamydia (C.) pneumoniae and used to screen the C. pneumoniae genome for vaccine candidates by expression library immunization (ELI). Biolistic delivery of genetic vaccine constructs elicited Th2-like immunity that was associated with inefficient elimination of C. pneumoniae. Delivery by injection elicited protective Th1-like responses. Since biolistic delivery of pools of ORFs was used in first round screening, the screen presumably selected against potent immunogens. Nevertheless, it was sufficiently accurate to identify three weakly protective antigens among all putative C. pneumoniae ORFs. The results suggest ELI discovery of highly protective C. pneumoniae vaccine candidates requires tight control of the Th1 immunity elicited by the genetically delivered library of test antigens.
一种近交A/J小鼠呼吸道攻击模型被验证可用于针对肺炎衣原体的疫苗测试，并通过表达文库免疫（ELI）用于筛选肺炎衣原体基因组中的疫苗候选物。基因疫苗构建体的生物弹道递送引发了类似Th2的免疫反应，这与肺炎衣原体的低效清除有关。注射递送引发了保护性的类似Th1的反应。由于在第一轮筛选中使用了ORF池的生物弹道递送，该筛选可能排除了强效免疫原。然而，它足以准确地在所有假定的肺炎衣原体ORF中鉴定出三种弱保护性抗原。结果表明，通过ELI发现高度保护性的肺炎衣原体疫苗候选物需要严格控制由基因递送的测试抗原文库引发的Th1免疫。
