Grau C, Overgaard J
Department of Experimental Clinical Oncology, Radiumstationen, Aarhus C., Denmark.
Cancer Chemother Pharmacol. 1992;30(4):277-80. doi: 10.1007/BF00686295.
The effect of etoposide (VP16), carmustine (BCNU), vincristine (VCR), methotrexate (MTX), and 5-fluorouracil (5-FU) on the oxic and hypoxic cells in a C3H mammary carcinoma in CDF1 mice was investigated using an in situ local-tumor-control (TCD50) assay. The surviving fraction (SF) was calculated from the size of the radiation dose needed to inactivate the surviving tumor cells in drug-treated tumors relative to untreated controls. Preferential drug cytotoxicity towards oxic and hypoxic cells was evaluated from the difference in the response to irradiation under ambient and clamped conditions, respectively. Three drugs caused a significant (P less than 0.05) reduction in the survival of hypoxic cells, the SFs being 0.31 (VP-16), 0.13 (BCNU) and 0.16 (VCR). VCR was also toxic towards oxic cells (SF, 0.17), whereas VP16 and BCNU had no significant effect on these cells (SF, 0.5 and 0.76, respectively). Two drugs produced significant killing of cells in the oxic compartment: 5-FU (SF, 0.10) and MTX (SF, 0.22); these two drugs had no effect on hypoxic cells (SF, 0.78 and 1.11, respectively).
使用原位局部肿瘤控制（TCD50）试验，研究了依托泊苷（VP16）、卡莫司汀（BCNU）、长春新碱（VCR）、甲氨蝶呤（MTX）和5-氟尿嘧啶（5-FU）对CDF1小鼠C3H乳腺癌中含氧细胞和缺氧细胞的影响。存活分数（SF）通过计算药物处理肿瘤中使存活肿瘤细胞失活所需的辐射剂量大小与未处理对照相比得出。分别根据在环境条件和钳夹条件下对辐射的反应差异，评估药物对含氧细胞和缺氧细胞的优先细胞毒性。三种药物导致缺氧细胞存活率显著降低（P小于0.05），存活分数分别为0.31（VP-16）、0.13（BCNU）和0.16（VCR）。VCR对含氧细胞也有毒性（存活分数为0.17），而VP16和BCNU对这些细胞无显著影响（存活分数分别为0.5和0.76）。两种药物对含氧区室的细胞有显著杀伤作用：5-FU（存活分数为0.10）和MTX（存活分数为0.22）；这两种药物对缺氧细胞无影响（存活分数分别为0.78和1.11）。
