Hernández Raquel, Martínez-Lara Esther, Cañuelo Ana, del Moral Maria Luisa, Blanco Santos, Siles Eva, Jiménez Ana, Pedrosa Juan Angel, Peinado Maria Angeles
Department of Experimental Biology, University of Jaén, Paraje Las Lagunillas s/n, E-23071 Jaén, Spain.
World J Gastroenterol. 2005 Dec 21;11(47):7480-5. doi: 10.3748/wjg.v11.i47.7480.
To analyze the relationship between perisinusoidal stellate cell (PSC) activation and the dietary fat quantity and composition in the treatment of hepatic steatosis.
Using an experimental rat model of steatosis based on the intake of a hyperlipidic diet (14% fat as olive oil or sunflower oil, HL-O and HL-S, respectively), we analyzed the liver's capability of recovery after the treatment with a normal-lipidic diet (5% fat as olive oil or sunflower oil, NL-O and NL-S, respectively) by immunocytochemical and Western blot analysis of glial fibrillary acidic protein (GFAP) expression in PSCs, collagen quantification and serum aminotransferase determination.
The fatty infiltration in the steatotic livers decreased after the treatment with both NL diets, indicating liver recovery. This decrease was accompanied with a lower collagen deposition and aminotransferase level as well as changes in the PSC population that increased the GFAP expression. The above-mentioned effects were more pronounced in animals fed on NL-O based diet.
Treatment with a balanced diet enriched in olive oil contributes to the liver recovery from a steatotic process. The PSC phenotype is a marker of this hepatic-recovery model.
分析肝窦周星状细胞（PSC）激活与饮食中脂肪含量及成分在肝脂肪变性治疗中的关系。
利用基于高脂饮食（分别以橄榄油或葵花籽油提供14%的脂肪，即HL - O和HL - S）摄入的实验性大鼠脂肪变性模型，通过对PSC中胶质纤维酸性蛋白（GFAP）表达进行免疫细胞化学和蛋白质印迹分析、胶原蛋白定量以及血清转氨酶测定，分析用正常脂质饮食（分别以橄榄油或葵花籽油提供5%的脂肪，即NL - O和NL - S）治疗后肝脏的恢复能力。
两种正常脂质饮食治疗后，脂肪变性肝脏中的脂肪浸润减少，表明肝脏恢复。这种减少伴随着较低的胶原蛋白沉积和转氨酶水平，以及PSC群体的变化，GFAP表达增加。上述效应在以NL - O为基础饮食喂养的动物中更为明显。
富含橄榄油的均衡饮食治疗有助于肝脏从脂肪变性过程中恢复。PSC表型是这种肝脏恢复模型的一个标志物。
