一种便捷的胸苷二醇亚磷酰胺单体的合成及其位点特异性掺入DNA片段
Synthesis of a convenient thymidine glycol phosphoramidite monomer and its site-specific incorporation into DNA fragments.
作者信息
Gasparutto Didier, Cognet Sonia, Roussel Solveig, Cadet Jean
机构信息
Labratoire des Lésions des Acides Nucléiques, Service Chimie Inorganique Biologique, UMR-E3 CEA-UJF, DRFMC, CEA Grenoble, France.
出版信息
Nucleosides Nucleotides Nucleic Acids. 2005;24(10-12):1831-42. doi: 10.1080/15257770500267279.
An original phosphoramidite building block of the thymidine glycol lesion has been prepared taking into account the additional diol function and the high lability of this oxidatively induced nucleobase damage. Then the modified nucleoside was site-specifically inserted into DNA fragments by solid support assembling followed by a "one-step" mild final deprotection treatment.
考虑到胸苷二醇损伤的额外二醇功能以及这种氧化诱导的核碱基损伤的高不稳定性，已经制备了胸苷二醇损伤的原始亚磷酰胺砌块。然后，通过固相支持物组装，将修饰的核苷位点特异性地插入到DNA片段中，随后进行“一步”温和的最终脱保护处理。
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