Efimov Vladimir A, Chakhmakhcheva Oksana G, Wickstrom Eric
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia.
Nucleosides Nucleotides Nucleic Acids. 2005;24(10-12):1853-74. doi: 10.1080/15257770500268830.
Negatively charged DNA mimics containing phosphonate analogoues of peptide nucleic acids were designed, and their physicochemical and biological properties were evaluated in the comparison with natural oligonucleotides, classical peptide nucleic acids, and morpholino phosphorodiamidate oligonucleotide analogues. The results obtained revealed a high potential of phosphonate-containing PNA derivatives for a number of biological applications, such as diagnostic, nucleic acids analysis, and inhibition of gene expression.
设计了含有肽核酸膦酸酯类似物的带负电荷的DNA模拟物，并将其物理化学和生物学性质与天然寡核苷酸、经典肽核酸和吗啉代磷酰胺寡核苷酸类似物进行了比较评估。所得结果表明含膦酸酯的PNA衍生物在许多生物学应用中具有很高的潜力，如诊断、核酸分析和基因表达抑制。
