Narberhaus Franz, Waldminghaus Torsten, Chowdhury Saheli
Lehrstuhl für Biologie der Mikroorganismen, Ruhr-Universität Bochum, Bochum, Germany.
FEMS Microbiol Rev. 2006 Jan;30(1):3-16. doi: 10.1111/j.1574-6976.2005.004.x.
Temperature is an important parameter that free-living cells monitor constantly. The expression of heat-shock, cold-shock and some virulence genes is coordinated in response to temperature changes. Apart from protein-mediated transcriptional control mechanisms, translational control by RNA thermometers is a widely used regulatory strategy. RNA thermometers are complex RNA structures that change their conformation in response to temperature. Most, but not all, RNA thermometers are located in the 5'-untranslated region and mask ribosome-binding sites by base pairing at low temperatures. Melting of the structure at increasing temperature permits ribosome access and translation initiation. Different cis-acting RNA thermometers and a trans-acting thermometer will be presented.
温度是自由生活细胞不断监测的一个重要参数。热休克、冷休克和一些毒力基因的表达会根据温度变化进行协调。除了蛋白质介导的转录控制机制外，RNA温度计介导的翻译控制是一种广泛使用的调控策略。RNA温度计是复杂的RNA结构，会随温度变化而改变其构象。大多数（但不是全部）RNA温度计位于5'非翻译区，在低温下通过碱基配对掩盖核糖体结合位点。随着温度升高，结构解链，使核糖体能够接近并启动翻译。本文将介绍不同的顺式作用RNA温度计和一种反式作用温度计。
