Tagawa Tetsuzo, Yoshino Ichiro, Yamaguchi Masafumi, Osoegawa Atsushi, Kameyama Toshifumi, Fukuyama Seiichi, Kometani Takuro, Maehara Yoshihiko
Department of Surgery and Science, Graduate School of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan.
Surg Today. 2006;36(2):135-9. doi: 10.1007/s00595-005-3132-2.
We studied the inhibitory effects of hypotonic cisplatin on the growth of malignant pleural mesothelioma (MPM) cell lines in vitro, and assessed the effectiveness of intraoperative intrapleural hypotonic cisplatin treatment combined with extrapleural pneumonectomy for patients with this tumor.
In the in vitro experiments, mesothelioma cell lines were exposed to various concentrations of cisplatin in either saline solution or distilled water for up to 5 min. After 48 h incubation, we calculated the inhibition of cell growth. In the clinical study, five patients with MPM underwent intraoperative intrapleural hypotonic cisplatin treatment combined with extrapleural pneumonectomy.
The hypotonic cisplatin treatment inhibited cell growth at a significantly greater rate than the isotonic cisplatin treatment. Just 1-5 min exposure to 10 microg/ml of hypotonic cisplatin inhibited growth by more than 80%. Clinically, no recurrence was found in four of the five patients after a median follow-up period of 27 months (range: 16-36 months), although contralateral multiple pulmonary metastases were found in one patient 10 months after surgery.
Hypotonic cisplatin treatment is effective against MPM, and should be investigated further.
我们研究了低渗顺铂对恶性胸膜间皮瘤（MPM）细胞系体外生长的抑制作用，并评估了术中胸膜腔内注入低渗顺铂联合胸膜外全肺切除术治疗该肿瘤患者的有效性。
在体外实验中，将间皮瘤细胞系暴露于不同浓度的顺铂溶液（生理盐水或蒸馏水）中长达5分钟。孵育48小时后，计算细胞生长抑制率。在临床研究中，5例MPM患者接受了术中胸膜腔内注入低渗顺铂联合胸膜外全肺切除术。
低渗顺铂治疗比等渗顺铂治疗对细胞生长的抑制率显著更高。仅1 - 5分钟暴露于10微克/毫升的低渗顺铂中，细胞生长抑制率就超过80%。临床上，5例患者中有4例在中位随访期27个月（范围：16 - 36个月）后未发现复发，尽管有1例患者在术后10个月出现对侧多发性肺转移。
低渗顺铂治疗对MPM有效，应进一步研究。
