Carbone Martina N, Etzel Mark R
Department of Chemical and Biological Engineering, University of Wisconsin, 1415 Engineering Drive, Madison, USA.
Biotechnol Bioeng. 2006 Apr 20;93(6):1221-4. doi: 10.1002/bit.20813.
The kinetics of lysozyme crystallization under seeded isothermal batch conditions was followed by measurement of the decline in solution concentration versus time. Kinetics were measured for five different values of the seed crystal mass. The data were analyzed using a recently proposed mathematical model. For each seed mass, the model fit the kinetic data well. Growth rate constants determined using the model were approximately constant over a sixfold increase in the seed crystal mass, and fell well within the range of values reported in the literature, but obtained using entirely different experimental techniques. These results confirmed the utility of the proposed model. The proposed model can be used to analyze crystallization kinetics using absorbance measurements only, without the need to characterize the crystal size, thus avoiding the need for expensive laser light scattering and digital microscopy instrumentation. Thus, the model offers a low-cost straightforward method to analyze and simulate the effects of changes in operating parameters such as the seed crystal mass, solution volume, initial protein concentration, pH, temperature, salt concentration, and time.
在晶种等温间歇条件下，通过测量溶液浓度随时间的下降来跟踪溶菌酶结晶的动力学。针对五种不同的晶种质量值测量了动力学。使用最近提出的数学模型对数据进行了分析。对于每种晶种质量，该模型都能很好地拟合动力学数据。使用该模型确定的生长速率常数在晶种质量增加六倍的范围内大致恒定，并且完全落在文献报道的值范围内，但采用的是完全不同的实验技术。这些结果证实了所提出模型的实用性。所提出的模型可仅使用吸光度测量来分析结晶动力学，而无需表征晶体尺寸，从而无需昂贵的激光散射和数字显微镜仪器。因此，该模型提供了一种低成本的直接方法来分析和模拟操作参数变化的影响，如晶种质量、溶液体积、初始蛋白质浓度、pH值、温度、盐浓度和时间。
