Dupeyrón D, González M, Sáez V, Ramón J, Rieumont J
Instituto de Materiales y Reactivos, Universidad de la Habana, Zapata y G, Vedado, Ciudad Havana, Cuba.
IEE Proc Nanobiotechnol. 2005 Oct;152(5):165-8. doi: 10.1049/ip-nbt:20050005.
In this preliminary work, an enteric polymer has been used for encapsulating bovine serum albumine (BSA) as a model protein drug. Poly (lactide-co- glycolide) has been commonly used for oral administration purposes as a polymer matrix, but in this case an enteric polymer was used effectively to protect the protein in a gastric environment. A modified water/oil/water technique was used to decrease the particle diameter, and transmission electron microscopy experiments showed that the average diameter of the nanoparticles obtained was below 100 nm. The spherical nature of the particles and their diameters strongly depend on the control of the process parameters. The encapsulation efficiency was 77% for sample B4, and protein release profiles for both samples B3 and B4 indicate that these systems possess controlled-release characteristics. Finally, as a result of electrophoresis (SDS-PAGE), the BSA was not chemically affected under encapsulation conditions.
在这项初步研究中，一种肠溶聚合物被用于包裹牛血清白蛋白（BSA）作为模型蛋白药物。聚（丙交酯-共-乙交酯）通常作为聚合物基质用于口服给药目的，但在本研究中，一种肠溶聚合物被有效地用于在胃部环境中保护蛋白质。采用改良的水/油/水技术来减小粒径，透射电子显微镜实验表明，所获得的纳米颗粒的平均直径低于100 nm。颗粒的球形性质及其直径在很大程度上取决于工艺参数的控制。样品B4的包封率为77%，样品B3和B4的蛋白质释放曲线表明这些体系具有控释特性。最后，通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）结果表明，BSA在包封条件下未受到化学影响。
