Jahanshahi M, Zhang Z, Lyddiatt A
Department of Chemical Engineering, University of Mazandaran, Faculty of Engineering, PO Box 484, Babol, Iran.
IEE Proc Nanobiotechnol. 2005 Jun;152(3):121-6. doi: 10.1049/ip-nbt:20045004.
A novel polymer-coated adsorbent (subtractive adsorbent) has been manufactured and evaluated for the recovery of nanoparticle bioproducts. The core principle was to coat inert macroporous polymers (e.g. agarose) upon adsorbent beads of varied ligands. Here BSA nanoparticles, with an average nanoparticle diameter 95 nm, were fabricated and selected as feedstock for the demonstration of the principle. The adsorption of a mixture of fluorescently labelled BSA solution and BSA nanoparticles was investigated in a batch binding experiment upon polymer-coated Streamline DEAE and visualised by laser scanning confocal microscopy. The mechanistic design of such adsorbents and their basic application for the recovery of target nano-bioproducts from complex feedstock is strongly indicated.
一种新型聚合物包覆吸附剂（减法吸附剂）已被制备并评估用于纳米颗粒生物产品的回收。其核心原理是在各种配体的吸附剂珠粒上包覆惰性大孔聚合物（如琼脂糖）。在此，制备了平均纳米颗粒直径为95nm的牛血清白蛋白（BSA）纳米颗粒，并将其选作原理验证的原料。在聚合物包覆的Streamline DEAE上进行批量结合实验，研究了荧光标记的BSA溶液和BSA纳米颗粒混合物的吸附情况，并通过激光扫描共聚焦显微镜进行可视化观察。强烈表明了此类吸附剂的机械设计及其从复杂原料中回收目标纳米生物产品的基本应用。
