Muirhead R W, Collins R P, Bremer P J
Department of Food Science, Otago University, Dunedin, New Zealand.
Lett Appl Microbiol. 2006 Feb;42(2):83-7. doi: 10.1111/j.1472-765X.2005.01823.x.
To investigate the number of Escherichia coli in runoff derived directly from fresh cowpats and to determine if the E. coli are attached to dense particles, in flocs or as individual cells.
Three cowpats were collected monthly from the same farm for 13 months and the number of E. coli in them estimated. A rainfall simulator was used to generate runoff from the individual cowpats, which was fractioned to determine the transported state of any E. coli present. The number of E. coli in the cowpat runoff was highly variable and was strongly correlated with the number of E. coli in the cowpat. Only a small percentage (approx. 8%) of the E. coli in runoff were attached to dense (>1.3 g ml(-1)) particles and there was no evidence of flocculation of the cells.
Escherichia coli in runoff from cowpats are transported predominantly as individual cells.
Mitigation strategies to reduce the number of faecal bacteria in overland flow from agricultural land need to be designed to trap single bacterial cells.
调查直接源自新鲜牛粪的径流中大肠杆菌的数量，并确定大肠杆菌是以附着在致密颗粒上、存在于絮凝物中还是以单个细胞的形式存在。
在13个月的时间里，每月从同一农场收集三块牛粪，并估算其中大肠杆菌的数量。使用降雨模拟器从单个牛粪中产生径流，将径流进行分级以确定其中存在的任何大肠杆菌的传输状态。牛粪径流中大肠杆菌的数量变化很大，且与牛粪中大肠杆菌的数量密切相关。径流中只有一小部分（约8%）大肠杆菌附着在致密（>1.3 g/ml）颗粒上，且没有细胞絮凝的迹象。
牛粪径流中的大肠杆菌主要以单个细胞的形式传输。
需要设计减少农田地表径流中粪便细菌数量的缓解策略，以捕获单个细菌细胞。
