Pillay T S, Makgoba M W
Department of Chemical Pathology, Royal Postgraduate Medical School, University of London, Hammersmith Hospital, UK.
FEBS Lett. 1992 Aug 10;308(1):38-42. doi: 10.1016/0014-5793(92)81045-n.
Sodium selenate stimulated tyrosine phosphorylation of the epidermal growth factor (EGF) receptor in A431 cells and enhanced the tyrosine phosphorylation of endogenous proteins in response to EGF in A431 cells and insulin in NIH 3T3 HIR3.5 cells. These effects occurred without changes in ligand binding, were not abolished by mercaptoethanol in the case of the EGF receptor, and appeared distinct from the effects of vanadate. These results support a role for selenium or selenoproteins in regulating EGF and insulin receptor tyrosine kinase activity and suggest a mechanism whereby selenium-containing compounds contribute to cell growth.
硒酸钠可刺激A431细胞中表皮生长因子（EGF）受体的酪氨酸磷酸化，并增强A431细胞中内源性蛋白质对EGF以及NIH 3T3 HIR3.5细胞中胰岛素的酪氨酸磷酸化反应。这些作用在配体结合未发生变化的情况下出现，对于EGF受体而言，巯基乙醇并不能消除这些作用，且这些作用似乎与钒酸盐的作用不同。这些结果支持了硒或硒蛋白在调节EGF和胰岛素受体酪氨酸激酶活性中发挥作用，并提示了含硒化合物促进细胞生长的一种机制。
